Suppression of polyclonal, tumor cell and alloantigen-induced proliferation: Identification of cycloxygenase pathway dependent and independent mechanisms

Kevin T. Hogan, Michael A. Hollingsworth, Robert E. Seymour, Marshall K. Quinn, Donald L. Evans

Research output: Contribution to journalArticle

Abstract

Polyclonal T cell activation, syngeneic tumor cell and alloantigen-induced proliferative responses were studied to determine if the regulation of these responses in normal and tumor-bearing NBR rats is mediated through products of the cyclooxygenase pathway and prostaglandin E2 (PGE2) in particular. Young rats and tumor-bearing rats have previously been shown to produce poor proliferative responses to PHA, Con A and syngeneic methylcholanthrene (MCA)-induced fibrosarcoma cells. The poor responses to PHA and Con A are mediated by PGE2 in unfractioned (UNF) and nylon wool adherent (ADH) cells. The same relationship was also established in the mixed leukocyte-tumor cell (MLTC) response to MCA tumor cells although it appears to be of only minor significance as the enhancement following indomethacin (IND) treatment is still relatively poor response. Indomethacin generally had no effect on the proliferative responses of tumor-bearing animals indicating that the suppression was not mediated through the cyclooxygenase pathway. We have also extended a previous observation in which UNF cells were found to be unresponsive to alloantigen stimulation. This suppression does not appear to be mediated through cyclooxygenase products as IND treatment does not enhance the UNF response although it does enhance the ADH response. These data indicate that a complex network of cyclooxygenase dependent and independent regulation exists in normal and tumor-bearing NBR rats.

Original languageEnglish (US)
Pages (from-to)49-57
Number of pages9
JournalImmunopharmacology
Volume7
Issue number1
DOIs
StatePublished - Feb 1984

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Isoantigens
Prostaglandin-Endoperoxide Synthases
Neoplasms
Indomethacin
Methylcholanthrene
Dinoprostone
Wool
Fibrosarcoma
Nylons
Leukocytes
Observation
T-Lymphocytes

Keywords

  • Indomethacin
  • Lymphoproliferation
  • Prostaglandin E
  • Salicylic acid
  • Suppression

ASJC Scopus subject areas

  • Pharmacology

Cite this

Suppression of polyclonal, tumor cell and alloantigen-induced proliferation : Identification of cycloxygenase pathway dependent and independent mechanisms. / Hogan, Kevin T.; Hollingsworth, Michael A.; Seymour, Robert E.; Quinn, Marshall K.; Evans, Donald L.

In: Immunopharmacology, Vol. 7, No. 1, 02.1984, p. 49-57.

Research output: Contribution to journalArticle

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