Suppressed impact of nitric oxide on renal arteriolar function in rats with chronic heart failure

Hideki Ikenaga, Naohito Ishii, Sean P. Didion, Kun Zhang, Kurtis G. Cornish, Kaushik P Patel, William Mayhan, Pamela K Carmines

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Abstract

We performed experiments to test the hypothesis that experimental heart failure (HF) is associated with altered nitric oxide (NO)-dependent influences on the renal microvasculature, including diminished modulation of constrictor responses to ANG II. Eight to ten weeks after inducing HF in rats by coronary artery ligation, we administered enalaprilat to suppress ANG II synthesis and studied renal arteriolar function using the in vitro blood- perfused juxtamedullary nephron technique. In kidneys from sham-operated rats, NO synthase inhibition [100 μM N(omega)-nitro-L-arginine (L-NNA)] reduced afferent arteriolar diameter by 4.1 ± 0.6 μm and enhanced ANG II responsiveness (10 nM ANG II decreased afferent diameter by 10.1 ± 1.4 μm before and 12.8 ± 1.6 μm during L-NNA treatment; P < 0.05). In kidneys from HF rats, L-NNA did not alter afferent arteriolar baseline diameter or ANG II responsiveness (10 nM ANG II decreased diameter by 12.5 ± 1.5 μm before and 12.5 ± 2.3 μm during L-NNA). The effects of L-NNA on efferent arteriolar function were also abated in HF rats. In renal cortex of HF rats, NO synthase activity was decreased by 63% and superoxide dismutase activity was diminished by 39% relative to tissue from sham-operated rats. Urinary nitrate/nitrite excretion was also reduced in HF rats. Thus both diminished synthesis and augmented degradation are likely to contribute to a decreased renal microvascular impact of endogenous NO during chronic HF, the consequences of which include loss of NO-dependent modulation of ANG II- induced vasoconstriction.

Original languageEnglish (US)
Pages (from-to)F79-F87
JournalAmerican Journal of Physiology - Renal Physiology
Volume276
Issue number1 45-1
StatePublished - Jan 1 1999

Fingerprint

Nitric Oxide
Heart Failure
Kidney
Nitric Oxide Synthase
Enalaprilat
Nitroarginine
Nephrons
Nitrites
Microvessels
Vasoconstriction
Nitrates
Superoxide Dismutase
Renal Insufficiency
Ligation
Coronary Vessels

Keywords

  • Angiotensin II
  • Myocardial infarction
  • Nitric oxide synthase
  • Renal microvasculature
  • Superoxide dismutase

ASJC Scopus subject areas

  • Physiology
  • Urology

Cite this

Suppressed impact of nitric oxide on renal arteriolar function in rats with chronic heart failure. / Ikenaga, Hideki; Ishii, Naohito; Didion, Sean P.; Zhang, Kun; Cornish, Kurtis G.; Patel, Kaushik P; Mayhan, William; Carmines, Pamela K.

In: American Journal of Physiology - Renal Physiology, Vol. 276, No. 1 45-1, 01.01.1999, p. F79-F87.

Research output: Contribution to journalArticle

Ikenaga, Hideki ; Ishii, Naohito ; Didion, Sean P. ; Zhang, Kun ; Cornish, Kurtis G. ; Patel, Kaushik P ; Mayhan, William ; Carmines, Pamela K. / Suppressed impact of nitric oxide on renal arteriolar function in rats with chronic heart failure. In: American Journal of Physiology - Renal Physiology. 1999 ; Vol. 276, No. 1 45-1. pp. F79-F87.
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