Supported lipid bilayers for atomic force microscopy studies

Zhengjian Lv, Siddhartha Banerjee, Karen Zagorski, Yuri L. Lyubchenko

Research output: Chapter in Book/Report/Conference proceedingChapter

8 Scopus citations

Abstract

Nanoimaging methods, atomic force microscopy (AFM) in particular, are widely used to study the interaction of biological molecules with the supported lipid bilayer (SLB), which itself is a traditional model for cellular membranes. Success in these studies is based on the availability of a stable SLB for the required observation period, which can extend several hours. The application of AFM requires that the SLB have a smooth morphology, thus enabling visualization of proteins and other molecules on its surface. Herein, we describe protocols for SLB assembly by using 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) and 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-l-serine (POPS) on a mica support. Our methodology enables us to assemble defect-free POPC and POPS SLBs that remain stable for at least 8 h. The application of such smooth and stable surfaces is illustrated by monitoring of the on-surface aggregation of amyloid proteins with the use of time-lapse AFM.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages129-143
Number of pages15
DOIs
StatePublished - Jan 1 2018

Publication series

NameMethods in Molecular Biology
Volume1814
ISSN (Print)1064-3745

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Keywords

  • Amyloid aggregation
  • Atomic force microscope
  • Nanoimaging
  • Supported lipid bilayer
  • Time-lapse imaging

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Lv, Z., Banerjee, S., Zagorski, K., & Lyubchenko, Y. L. (2018). Supported lipid bilayers for atomic force microscopy studies. In Methods in Molecular Biology (pp. 129-143). (Methods in Molecular Biology; Vol. 1814). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-8591-3_8