Superoxide mediates sympathoexcitation in heart failure

Roles of angiotensin II and NAD(P)H oxidase

Lie Gao, Wei Wang, Yulong Li, Harold D Schultz, Dongmei Liu, Kurtis G. Cornish, Irving H Zucker

Research output: Contribution to journalArticle

198 Citations (Scopus)

Abstract

Chronic heart failure (CHF) is often associated with excitation of the sympathetic nervous system. This event is thought to be a negative predictor of survival in CHF. Sympathoexcitation and central angiotensin II (Ang II) have been causally linked. Recent studies have shown that NAD(P)H oxidase-derived reactive oxidant species (ROS) are important mediators of Ang II signaling. In the present study, we tested the hypothesis that central Ang II activates sympathetic outflow by stimulation of NAD(P)H oxidase and ROS in the CHF state. CHF was induced in male New Zealand White rabbits by chronic ventricular tachycardia. Using radio telemetry of arterial pressure and intracerebroventricular infusions, experiments were performed in the conscious state. Renal sympathetic nerve activity (RSNA) was recorded as a direct measure of sympathetic outflow. Intracerebroventricular Ang II significantly increased RSNA in sham (131.5±13.3% of control) and CHF (193.6±11.9% of control) rabbits. The increase in CHF rabbits was significantly greater than in sham rabbits (P<0.01). These responses were abolished by intracerebroventricular losartan, tempol, or apocynin. Resting RSNA was significantly reduced by intracerebroventricular losartan, tempol, or apocynin in CHF rabbits but not in sham rabbits. Intracerebroventricular administration of the Superoxide dismutase inhibitor diethyldithio-carbamic acid increased RSNA significantly more in sham compared with CHF rabbits. NADPH-dependent Superoxide anion production in the rostral ventrolateral medulla (RVLM) was increased by 2.9-fold in CHF rabbits compared with sham rabbits. Finally, increases in the RVLM mRNA and protein expression of Ang II type 1 (AT 1) receptor and subunits of NAD(P)H oxidase (p40phox, p47phox, and gp91phox) were demonstrated in CHF rabbits. These data demonstrate intense radical stress in autonomic areas of the brain in experimental CHF and provide evidence for a tight relationship between Ang II and ROS as contributors to Sympathoexcitation in CHF.

Original languageEnglish (US)
Pages (from-to)937-944
Number of pages8
JournalCirculation Research
Volume95
Issue number9
DOIs
StatePublished - Oct 29 2004

Fingerprint

NADPH Oxidase
Superoxides
Angiotensin II
Heart Failure
Rabbits
Oxidants
Kidney
Losartan
Intraventricular Infusions
Telemetry
Angiotensin Type 1 Receptor
Sympathetic Nervous System
Ventricular Tachycardia
Radio
NADP
Superoxide Dismutase
Arterial Pressure

Keywords

  • Angiotensin receptors
  • Free radicals
  • RVLM
  • Ventricular pacing

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Superoxide mediates sympathoexcitation in heart failure : Roles of angiotensin II and NAD(P)H oxidase. / Gao, Lie; Wang, Wei; Li, Yulong; Schultz, Harold D; Liu, Dongmei; Cornish, Kurtis G.; Zucker, Irving H.

In: Circulation Research, Vol. 95, No. 9, 29.10.2004, p. 937-944.

Research output: Contribution to journalArticle

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abstract = "Chronic heart failure (CHF) is often associated with excitation of the sympathetic nervous system. This event is thought to be a negative predictor of survival in CHF. Sympathoexcitation and central angiotensin II (Ang II) have been causally linked. Recent studies have shown that NAD(P)H oxidase-derived reactive oxidant species (ROS) are important mediators of Ang II signaling. In the present study, we tested the hypothesis that central Ang II activates sympathetic outflow by stimulation of NAD(P)H oxidase and ROS in the CHF state. CHF was induced in male New Zealand White rabbits by chronic ventricular tachycardia. Using radio telemetry of arterial pressure and intracerebroventricular infusions, experiments were performed in the conscious state. Renal sympathetic nerve activity (RSNA) was recorded as a direct measure of sympathetic outflow. Intracerebroventricular Ang II significantly increased RSNA in sham (131.5±13.3{\%} of control) and CHF (193.6±11.9{\%} of control) rabbits. The increase in CHF rabbits was significantly greater than in sham rabbits (P<0.01). These responses were abolished by intracerebroventricular losartan, tempol, or apocynin. Resting RSNA was significantly reduced by intracerebroventricular losartan, tempol, or apocynin in CHF rabbits but not in sham rabbits. Intracerebroventricular administration of the Superoxide dismutase inhibitor diethyldithio-carbamic acid increased RSNA significantly more in sham compared with CHF rabbits. NADPH-dependent Superoxide anion production in the rostral ventrolateral medulla (RVLM) was increased by 2.9-fold in CHF rabbits compared with sham rabbits. Finally, increases in the RVLM mRNA and protein expression of Ang II type 1 (AT 1) receptor and subunits of NAD(P)H oxidase (p40phox, p47phox, and gp91phox) were demonstrated in CHF rabbits. These data demonstrate intense radical stress in autonomic areas of the brain in experimental CHF and provide evidence for a tight relationship between Ang II and ROS as contributors to Sympathoexcitation in CHF.",
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AU - Gao, Lie

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AU - Li, Yulong

AU - Schultz, Harold D

AU - Liu, Dongmei

AU - Cornish, Kurtis G.

AU - Zucker, Irving H

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