Superinfection exclusion of alphaviruses in three mosquito cell lines persistently infected with Sindbis virus

Adam R Karpf, Edith Lenches, Ellen G. Strauss, James H. Strauss, Dennis T. Brown

Research output: Contribution to journalArticle

110 Citations (Scopus)

Abstract

Three Aedes albopictus (mosquito) cell lines persistently infected with Sindbis virus excluded the replication of both homologous (various strains of Sindbis) and heterologous (Aura, Semliki Forest, and Ross River) alphaviruses. In contrast, an unrelated flavivirus, yellow fever virus, replicated equally well in uninfected and persistently infected cells of each line. Sindbis virus and Semliki Forest virus are among the most distantly related alphaviruses, and our results thus indicate that mosquito cells persistently infected with Sindbis virus are broadly able to exclude other alphaviruses but that exclusion is restricted to members of the alphavirus genus. Superinfection exclusion occurred to the same extent in three biologically distinct cell clones, indicating that the expression of superinfection exclusion is conserved among A. albopictus cell types. Superinfection of persistently infected C7-10 cells, which show a severe cytopathic effect during primary Sindbis virus infection, by homologous virus does not produce cytopathology, consistent with the idea that cytopathology requires significant levels of viral replication. A possible model for the molecular basis of superinfection exclusion, which suggests a central role for the alphavirus trans-acting protease that processes the nonstructural proteins, is discussed in light of these results.

Original languageEnglish (US)
Pages (from-to)7119-7123
Number of pages5
JournalJournal of virology
Volume71
Issue number9
StatePublished - Sep 4 1997

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Sindbis virus
Sindbis Virus
Alphavirus
Superinfection
Culicidae
cell lines
Cell Line
Aedes albopictus
virus replication
Semliki Forest virus
Yellow fever virus
cells
Semliki forest virus
Flavivirus
molecular models
Molecular Models
cytopathogenicity
Aedes
Virus Diseases
Virus Replication

ASJC Scopus subject areas

  • Immunology

Cite this

Superinfection exclusion of alphaviruses in three mosquito cell lines persistently infected with Sindbis virus. / Karpf, Adam R; Lenches, Edith; Strauss, Ellen G.; Strauss, James H.; Brown, Dennis T.

In: Journal of virology, Vol. 71, No. 9, 04.09.1997, p. 7119-7123.

Research output: Contribution to journalArticle

Karpf, AR, Lenches, E, Strauss, EG, Strauss, JH & Brown, DT 1997, 'Superinfection exclusion of alphaviruses in three mosquito cell lines persistently infected with Sindbis virus', Journal of virology, vol. 71, no. 9, pp. 7119-7123.
Karpf, Adam R ; Lenches, Edith ; Strauss, Ellen G. ; Strauss, James H. ; Brown, Dennis T. / Superinfection exclusion of alphaviruses in three mosquito cell lines persistently infected with Sindbis virus. In: Journal of virology. 1997 ; Vol. 71, No. 9. pp. 7119-7123.
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