19F NMR quantitation of lens aldose reductase activity using 3-deoxy-3-fluoro-D-glucose

K. Karino, P. F. Kador, B. Berkowitz, R. S. Balaban

Research output: Contribution to journalArticle

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Abstract

In the present study we have determined the kinetics of 3-deoxy-3-fluoro-D-glucose (3-FG) as a substrate for the aldose reductase reaction in vitro. In addition, we compared the 3-deoxy-3-fluoro-sorbitol (3-FS) production rates from 3-FG in the intact lens using 19F NMR with conventional aldose reductase determinations in extracts from the same lenses. The affinity of in vitro aldose reductase for 3-FG was approximately 20 times greater (9.3 mM) than that for glucose (188 mM). An excellent correlation between the rate of 3-FS production in the intact canine lens, determined with 19F NMR, and extracted aldose reductase activity was observed. The relatively high affinity of aldose reductase for 3-FG and the correlation of 3-FS production with enzyme activity in the intact lens suggests that 3-FS production from 3-FG detected by 19F NMR could provide an accurate noninvasive determination of aldose reductase activity in the eye lens.

Original languageEnglish (US)
Pages (from-to)20970-20975
Number of pages6
JournalJournal of Biological Chemistry
Volume266
Issue number31
StatePublished - Jan 1 1991

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Aldehyde Reductase
Lenses
Sorbitol
Nuclear magnetic resonance
Glucose
Crystalline Lens
Enzyme activity
3-deoxy-3-fluoro-D-glucose
Canidae
Kinetics
Substrates
Enzymes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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19F NMR quantitation of lens aldose reductase activity using 3-deoxy-3-fluoro-D-glucose. / Karino, K.; Kador, P. F.; Berkowitz, B.; Balaban, R. S.

In: Journal of Biological Chemistry, Vol. 266, No. 31, 01.01.1991, p. 20970-20975.

Research output: Contribution to journalArticle

Karino, K. ; Kador, P. F. ; Berkowitz, B. ; Balaban, R. S. / 19F NMR quantitation of lens aldose reductase activity using 3-deoxy-3-fluoro-D-glucose. In: Journal of Biological Chemistry. 1991 ; Vol. 266, No. 31. pp. 20970-20975.
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