13C NMR reveals no evidence of n-π* interactions in proteins

Bradley Worley, Georgia Richard, Gerard Harbison, Robert Powers

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

An n-π* interaction between neighboring carbonyl groups has been postulated to stabilize protein structures. Such an interaction would affect the 13C chemical shielding of the carbonyl groups, whose paramagnetic component is dominated by n-π* and n-π* excitations. Model compound calculations indicate that both the interaction energetics and the chemical shielding of the carbonyl group are instead dominated by a classical dipole-dipole interaction. A set of high-resolution protein structures with associated carbonyl 13C chemical shift assignments verifies this correlation and provides no evidence for an inter-carbonyl n-π* interaction.

Original languageEnglish (US)
Article numbere42075
JournalPloS one
Volume7
Issue number8
DOIs
StatePublished - Aug 2 2012

Fingerprint

protein structure
Shielding
Nuclear magnetic resonance
Chemical shift
Proteins
proteins
Carbon-13 Magnetic Resonance Spectroscopy

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

13C NMR reveals no evidence of n-π* interactions in proteins. / Worley, Bradley; Richard, Georgia; Harbison, Gerard; Powers, Robert.

In: PloS one, Vol. 7, No. 8, e42075, 02.08.2012.

Research output: Contribution to journalArticle

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