Sumoylation differentially regulates Sp1 to control cell differentiation

Lili Gong, Wei Ke Ji, Xiao Hui Hu, Wen Feng Hu, Xiang Cheng Tang, Zhao Xia Huang, Ling Li, Mugen Liu, Shi-Hua Xiang, Erxi Wu, Zachary Woodward, Yi Zhi Liu, Quan Dong Nguyen, David Wan Cheng Li

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

The mammalian small ubiquitin-like modifiers (SUMOs) are actively involved in regulating differentiation of different cell types. However, the functional differences between SUMO isoforms and their mechanisms of action remain largely unknown. Using the ocular lens as a model system, we demonstrate that different SUMOs display distinct functions in regulating differentiation of epithelial cells into fiber cells. During lens differentiation, SUMO1 and SUMO2/3 displayed different expression, localization, and targets, suggesting differential functions. Indeed, overexpression of SUMO2/3, but not SUMO1, inhibited basic (b) FGF-induced cell differentiation. In contrast, knockdown of SUMO1, but not SUMO2/3, also inhibited bFGF action. Mechanistically, specificity protein 1 (Sp1), a major transcription factor that controls expression of lens-specific genes such as β-crystallins, was positively regulated by SUMO1 but negatively regulated by SUMO2. SUMO2 was found to inhibit Sp1 functions through several mechanisms: sumoylating it at K683 to attenuate DNA binding, and at K16 to increase its turnover. SUMO2 also interfered with the interaction between Sp1 and the coactivator, p300, and recruited a repressor, Sp3 to β-crystallin gene promoters, to negatively regulate their expression. Thus, stable SUMO1, but diminishing SUMO2/3, during lens development is necessary for normal lens differentiation. In support of this conclusion, SUMO1 and Sp1 formed complexes during early and later stages of lens development. In contrast, an interaction between SUMO2/3 and Sp1 was detected only during the initial lens vesicle stage. Together, our results establish distinct roles of different SUMO isoforms and demonstrate for the first time, to our knowledge, that Sp1 acts as a major transcription factor target for SUMO control of cell differentiation.

Original languageEnglish (US)
Pages (from-to)5574-5579
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume111
Issue number15
DOIs
StatePublished - Apr 15 2014

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Sumoylation
Lenses
Cell Differentiation
Ubiquitin
Crystallins
Proteins
Protein Isoforms
Transcription Factors
Crystalline Lens
Genes
Epithelial Cells
DNA

Keywords

  • Crystallin Gene Expression
  • Eye Development
  • Transcription Regulation

ASJC Scopus subject areas

  • General

Cite this

Sumoylation differentially regulates Sp1 to control cell differentiation. / Gong, Lili; Ji, Wei Ke; Hu, Xiao Hui; Hu, Wen Feng; Tang, Xiang Cheng; Huang, Zhao Xia; Li, Ling; Liu, Mugen; Xiang, Shi-Hua; Wu, Erxi; Woodward, Zachary; Liu, Yi Zhi; Nguyen, Quan Dong; Li, David Wan Cheng.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 111, No. 15, 15.04.2014, p. 5574-5579.

Research output: Contribution to journalArticle

Gong, L, Ji, WK, Hu, XH, Hu, WF, Tang, XC, Huang, ZX, Li, L, Liu, M, Xiang, S-H, Wu, E, Woodward, Z, Liu, YZ, Nguyen, QD & Li, DWC 2014, 'Sumoylation differentially regulates Sp1 to control cell differentiation', Proceedings of the National Academy of Sciences of the United States of America, vol. 111, no. 15, pp. 5574-5579. https://doi.org/10.1073/pnas.1315034111
Gong, Lili ; Ji, Wei Ke ; Hu, Xiao Hui ; Hu, Wen Feng ; Tang, Xiang Cheng ; Huang, Zhao Xia ; Li, Ling ; Liu, Mugen ; Xiang, Shi-Hua ; Wu, Erxi ; Woodward, Zachary ; Liu, Yi Zhi ; Nguyen, Quan Dong ; Li, David Wan Cheng. / Sumoylation differentially regulates Sp1 to control cell differentiation. In: Proceedings of the National Academy of Sciences of the United States of America. 2014 ; Vol. 111, No. 15. pp. 5574-5579.
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abstract = "The mammalian small ubiquitin-like modifiers (SUMOs) are actively involved in regulating differentiation of different cell types. However, the functional differences between SUMO isoforms and their mechanisms of action remain largely unknown. Using the ocular lens as a model system, we demonstrate that different SUMOs display distinct functions in regulating differentiation of epithelial cells into fiber cells. During lens differentiation, SUMO1 and SUMO2/3 displayed different expression, localization, and targets, suggesting differential functions. Indeed, overexpression of SUMO2/3, but not SUMO1, inhibited basic (b) FGF-induced cell differentiation. In contrast, knockdown of SUMO1, but not SUMO2/3, also inhibited bFGF action. Mechanistically, specificity protein 1 (Sp1), a major transcription factor that controls expression of lens-specific genes such as β-crystallins, was positively regulated by SUMO1 but negatively regulated by SUMO2. SUMO2 was found to inhibit Sp1 functions through several mechanisms: sumoylating it at K683 to attenuate DNA binding, and at K16 to increase its turnover. SUMO2 also interfered with the interaction between Sp1 and the coactivator, p300, and recruited a repressor, Sp3 to β-crystallin gene promoters, to negatively regulate their expression. Thus, stable SUMO1, but diminishing SUMO2/3, during lens development is necessary for normal lens differentiation. In support of this conclusion, SUMO1 and Sp1 formed complexes during early and later stages of lens development. In contrast, an interaction between SUMO2/3 and Sp1 was detected only during the initial lens vesicle stage. Together, our results establish distinct roles of different SUMO isoforms and demonstrate for the first time, to our knowledge, that Sp1 acts as a major transcription factor target for SUMO control of cell differentiation.",
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AU - Huang, Zhao Xia

AU - Li, Ling

AU - Liu, Mugen

AU - Xiang, Shi-Hua

AU - Wu, Erxi

AU - Woodward, Zachary

AU - Liu, Yi Zhi

AU - Nguyen, Quan Dong

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AB - The mammalian small ubiquitin-like modifiers (SUMOs) are actively involved in regulating differentiation of different cell types. However, the functional differences between SUMO isoforms and their mechanisms of action remain largely unknown. Using the ocular lens as a model system, we demonstrate that different SUMOs display distinct functions in regulating differentiation of epithelial cells into fiber cells. During lens differentiation, SUMO1 and SUMO2/3 displayed different expression, localization, and targets, suggesting differential functions. Indeed, overexpression of SUMO2/3, but not SUMO1, inhibited basic (b) FGF-induced cell differentiation. In contrast, knockdown of SUMO1, but not SUMO2/3, also inhibited bFGF action. Mechanistically, specificity protein 1 (Sp1), a major transcription factor that controls expression of lens-specific genes such as β-crystallins, was positively regulated by SUMO1 but negatively regulated by SUMO2. SUMO2 was found to inhibit Sp1 functions through several mechanisms: sumoylating it at K683 to attenuate DNA binding, and at K16 to increase its turnover. SUMO2 also interfered with the interaction between Sp1 and the coactivator, p300, and recruited a repressor, Sp3 to β-crystallin gene promoters, to negatively regulate their expression. Thus, stable SUMO1, but diminishing SUMO2/3, during lens development is necessary for normal lens differentiation. In support of this conclusion, SUMO1 and Sp1 formed complexes during early and later stages of lens development. In contrast, an interaction between SUMO2/3 and Sp1 was detected only during the initial lens vesicle stage. Together, our results establish distinct roles of different SUMO isoforms and demonstrate for the first time, to our knowledge, that Sp1 acts as a major transcription factor target for SUMO control of cell differentiation.

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