Sulfate concentrations and transport in human bronchial epithelial cells

N. K. Mohapatra, Pi-Wan Cheng, J. C. Parker, A. M. Paradiso, J. R. Yankaskas, R. C. Boucher, T. F. Boat

Research output: Contribution to journalArticle

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Abstract

Inorganic sulfate concentrations in the cytoplasm of human bronchial epithelial cells exceeded levels in the bathing medium under all circumstances tested. Cell sulfate concentrations were directly related to medium sulfate concentrations and inversely related to medium chloride concentrations. In physiological media there was a sulfate compartment of ~0.3 mM that exchanged very slowly with extracellular sulfate. In media lacking chloride, sulfate was accumulated by the cells to a level as high as 2 mM. Sulfate uptake was markedly inhibited by external chloride and by stilbene sulfonic acid derivatives but was not affected by sodium in the medium. Efflux of 35SO4/2- was stimulated by both chloride and sulfate in the bathing medium but inhibited by stilbenes. The following compounds had no effect on sulfate movements: phorbol esters, adenosine 3',5'-cyclic monophospate derivatives, and okadaic acid. Changes in medium tonicity were likewise without effect. Our results suggest that human bronchial epithelial cells maintain a steady-state disequilibrium for inorganic sulfate. Furthermore, sulfate appears to exist in at least two compartments in the cells: one that is slowly exchangeable with sulfate in the medium and another exchangeable compartment that is of negligible size in physiological media but that becomes very large in media lacking chloride. Sulfate is transported by an anion exchanger of broad specificity that is not influenced by substances known to modulate chloride channels.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Cell Physiology
Volume264
Issue number5 33-5
StatePublished - Jun 2 1993

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Sulfates
Epithelial Cells
Chlorides
Stilbenes
Derivatives
Okadaic Acid
Chloride Channels
Sulfonic Acids
Phorbol Esters
Adenosine
Anions
Cytoplasm
Sodium

Keywords

  • anion transport
  • chloride channels
  • cystic fibrosis

ASJC Scopus subject areas

  • Physiology
  • Cell Biology

Cite this

Mohapatra, N. K., Cheng, P-W., Parker, J. C., Paradiso, A. M., Yankaskas, J. R., Boucher, R. C., & Boat, T. F. (1993). Sulfate concentrations and transport in human bronchial epithelial cells. American Journal of Physiology - Cell Physiology, 264(5 33-5).

Sulfate concentrations and transport in human bronchial epithelial cells. / Mohapatra, N. K.; Cheng, Pi-Wan; Parker, J. C.; Paradiso, A. M.; Yankaskas, J. R.; Boucher, R. C.; Boat, T. F.

In: American Journal of Physiology - Cell Physiology, Vol. 264, No. 5 33-5, 02.06.1993.

Research output: Contribution to journalArticle

Mohapatra, NK, Cheng, P-W, Parker, JC, Paradiso, AM, Yankaskas, JR, Boucher, RC & Boat, TF 1993, 'Sulfate concentrations and transport in human bronchial epithelial cells', American Journal of Physiology - Cell Physiology, vol. 264, no. 5 33-5.
Mohapatra NK, Cheng P-W, Parker JC, Paradiso AM, Yankaskas JR, Boucher RC et al. Sulfate concentrations and transport in human bronchial epithelial cells. American Journal of Physiology - Cell Physiology. 1993 Jun 2;264(5 33-5).
Mohapatra, N. K. ; Cheng, Pi-Wan ; Parker, J. C. ; Paradiso, A. M. ; Yankaskas, J. R. ; Boucher, R. C. ; Boat, T. F. / Sulfate concentrations and transport in human bronchial epithelial cells. In: American Journal of Physiology - Cell Physiology. 1993 ; Vol. 264, No. 5 33-5.
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