Suggestive evidence for the induction of colonic aberrant crypts in mice fed sodium nitrite

Lin Zhou, Muhammad Zahid, Muhammad M. Anwar, Karen L. Pennington, Samuel Monroe Cohen, James Lowell Wisecarver, Valerie Shostrom, Sidney S. Mirvish

Research output: Contribution to journalArticle

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Abstract

A reported linkage between processed (nitrite-treated) meat products and the incidence of colon cancer could be due to sodium nitrite (NaNO2) itself or to N-nitroso compounds produced from the nitrite. Exposure to nitrite occurs due to residual nitrite in processed meat and to salivary nitrite arising by reduction of nitrate in vegetables and drinking water. Here we tested whether NaNO2 could induce colonic aberrant crypts (ABC) or ABC foci (ACF), which are putative precursors of colon cancer. We fed NaNO2 in drinking water for 20-25 wk to groups of 8-20 adult female mice. After sacrifice, ABC and ACF were counted in 2-cm distal colonic segments. In Experiment 1, no significant differences in ABC/ACF induction were seen between groups of 13-14 A/J mice fed 0, 0.5, or 1.0 g NaNO2/l drinking water. NaNO2 also did not affect fasting blood glucose levels. In Experiment 2, we fed 0, 1.0, 1.25, or 1.5 g NaNO2/l water to groups of 15 CF-1 mice. Five of the mice fed 1.5 g NaNO2/l showed ABC, whereas all other groups showed only 0-2 ABC/group, including 0 ABC for the group fed 1.25 g NaNO2/l. Overall statistical analysis indicated a dose-response p trends of 0.04. Pairwise comparison of ABC between groups fed 1.25 and 1.5 g NaNO2/l showed p 0.02 for both ABC and ACF, but a similar comparison between the untreated and 1.5 g/l groups showed no significant effects. In Experiment 3, hot dogs (18% of diet), which were fed to CF-1 mice previously treated with azoxymethane, inhibited ABC and ACF induction, but this effect was not significant (P = 0.10-0.12). In conclusion, these results support the view that NaNO2 may be a risk factor for colon carcinogenesis.

Original languageEnglish (US)
Pages (from-to)105-112
Number of pages8
JournalNutrition and cancer
Volume68
Issue number1
DOIs
StatePublished - Jan 2 2016

Fingerprint

Aberrant Crypt Foci
Sodium Nitrite
Nitrites
Drinking Water
Colonic Neoplasms
Azoxymethane
Nitroso Compounds
Meat Products
Vegetables
Nitrates
Meat
Blood Glucose
Fasting
Colon
Carcinogenesis
Dogs
Diet
Water
Incidence

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Oncology
  • Nutrition and Dietetics
  • Cancer Research

Cite this

Suggestive evidence for the induction of colonic aberrant crypts in mice fed sodium nitrite. / Zhou, Lin; Zahid, Muhammad; Anwar, Muhammad M.; Pennington, Karen L.; Cohen, Samuel Monroe; Wisecarver, James Lowell; Shostrom, Valerie; Mirvish, Sidney S.

In: Nutrition and cancer, Vol. 68, No. 1, 02.01.2016, p. 105-112.

Research output: Contribution to journalArticle

Zhou, Lin ; Zahid, Muhammad ; Anwar, Muhammad M. ; Pennington, Karen L. ; Cohen, Samuel Monroe ; Wisecarver, James Lowell ; Shostrom, Valerie ; Mirvish, Sidney S. / Suggestive evidence for the induction of colonic aberrant crypts in mice fed sodium nitrite. In: Nutrition and cancer. 2016 ; Vol. 68, No. 1. pp. 105-112.
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abstract = "A reported linkage between processed (nitrite-treated) meat products and the incidence of colon cancer could be due to sodium nitrite (NaNO2) itself or to N-nitroso compounds produced from the nitrite. Exposure to nitrite occurs due to residual nitrite in processed meat and to salivary nitrite arising by reduction of nitrate in vegetables and drinking water. Here we tested whether NaNO2 could induce colonic aberrant crypts (ABC) or ABC foci (ACF), which are putative precursors of colon cancer. We fed NaNO2 in drinking water for 20-25 wk to groups of 8-20 adult female mice. After sacrifice, ABC and ACF were counted in 2-cm distal colonic segments. In Experiment 1, no significant differences in ABC/ACF induction were seen between groups of 13-14 A/J mice fed 0, 0.5, or 1.0 g NaNO2/l drinking water. NaNO2 also did not affect fasting blood glucose levels. In Experiment 2, we fed 0, 1.0, 1.25, or 1.5 g NaNO2/l water to groups of 15 CF-1 mice. Five of the mice fed 1.5 g NaNO2/l showed ABC, whereas all other groups showed only 0-2 ABC/group, including 0 ABC for the group fed 1.25 g NaNO2/l. Overall statistical analysis indicated a dose-response p trends of 0.04. Pairwise comparison of ABC between groups fed 1.25 and 1.5 g NaNO2/l showed p 0.02 for both ABC and ACF, but a similar comparison between the untreated and 1.5 g/l groups showed no significant effects. In Experiment 3, hot dogs (18{\%} of diet), which were fed to CF-1 mice previously treated with azoxymethane, inhibited ABC and ACF induction, but this effect was not significant (P = 0.10-0.12). In conclusion, these results support the view that NaNO2 may be a risk factor for colon carcinogenesis.",
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