Substrate specificity, kinetic properties and inhibition by fumonisin B1of ceramide synthase isoforms from Arabidopsis

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Ceramide makes up the acyl-backbone of sphingolipids and plays a central role in determining the function of these essential membrane lipids. In Arabidopsis, the varied chemical composition of ceramide is determined by the specificity of three different isoforms of ceramide synthase, denoted LAG one homologue 1, -2 and -3 (LOH1, LOH2 and LOH3), for a range of long-chain base (LCB) and acyl-CoA substrates. The contribution of each of these isoforms to the synthesis of ceramide was investigated by in vitro ceramide synthase assays. The plant LCB phytosphingosine was efficiently used by the LOH1 and LOH3 isoforms, with LOH1 having the lowest Km for the LCB substrate of the three isoforms. In contrast, sphinganine was used efficiently only by the LOH2 isoform. Acyl-CoA specificity was also distinguished between the three isoforms with LOH2 almost completely specific for palmitoyl-CoA whereas the LOH1 isoform showed greatest activity with lignoceroyl- and hexacosanoyl-CoAs. Interestingly, unsaturated acyl-CoAs were not used efficiently by any isoform whereas unsaturated LCB substrates were preferred by LOH2 and 3. Fumonisin B1 (FB1 )is a general inhibitor of ceramide synthases but LOH1 was found to have a much lower Ki than the other isoforms pointing towards the origin of FB1 sensitivity in plants. Overall, the data suggest distinct roles and modes of regulation for each of the ceramide synthases in Arabidopsis sphingolipid metabolism.

Original languageEnglish (US)
Pages (from-to)593-603
Number of pages11
JournalBiochemical Journal
Volume473
Issue number5
DOIs
StatePublished - Mar 1 2016

Fingerprint

Fumonisins
Substrate Specificity
Arabidopsis
Protein Isoforms
Kinetics
Substrates
Ceramides
Acyl Coenzyme A
Sphingolipids
phytosphingosine
Palmitoyl Coenzyme A
dihydroceramide desaturase
Membrane Lipids
Metabolism
Assays

Keywords

  • Arabidopsis thaliana
  • Ceramide
  • Enzyme kinetics
  • Plant biochemistry
  • Sphingolipid

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Substrate specificity, kinetic properties and inhibition by fumonisin B1of ceramide synthase isoforms from Arabidopsis. / Luttgeharm, Kyle D.; Cahoon, Edgar B.; Markham, Jonathan E.

In: Biochemical Journal, Vol. 473, No. 5, 01.03.2016, p. 593-603.

Research output: Contribution to journalArticle

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