Substrate Pathways in the nitrogenase MoFe protein by experimental identification of small molecule binding sites

Christine N. Morrison, Julie A. Hoy, Limei Zhang, Oliver Einsle, Douglas C. Rees

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

In the nitrogenase molybdenum-iron (MoFe) protein, we have identified five potential substrate access pathways from the protein surface to the FeMo-cofactor (the active site) or the P-cluster using experimental structures of Xe pressurized into MoFe protein crystals from Azotobacter vinelandii and Clostridium pasteurianum. Additionally, all published structures of the MoFe protein, including those from Klebsiella pneumoniae, were analyzed for the presence of nonwater, small molecules bound to the protein interior. Each pathway is based on identification of plausible routes from buried small molecule binding sites to both the protein surface and a metallocluster. Of these five pathways, two have been previously suggested as substrate access pathways. While the small molecule binding sites are not conserved among the three species of MoFe protein, residues lining the pathways are generally conserved, indicating that the proposed pathways may be accessible in all three species. These observations imply that there is unlikely a unique pathway utilized for substrate access from the protein surface to the active site; however, there may be preferred pathways such as those described here.

Original languageEnglish (US)
Pages (from-to)2052-2060
Number of pages9
JournalBiochemistry
Volume54
Issue number11
DOIs
StatePublished - Mar 24 2015

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Molybdoferredoxin
Nitrogenase
Binding Sites
Molecules
Substrates
Membrane Proteins
Catalytic Domain
Clostridium
Molybdenum
Azotobacter vinelandii
Linings
Klebsiella pneumoniae
Iron
Crystals
Proteins

ASJC Scopus subject areas

  • Biochemistry

Cite this

Substrate Pathways in the nitrogenase MoFe protein by experimental identification of small molecule binding sites. / Morrison, Christine N.; Hoy, Julie A.; Zhang, Limei; Einsle, Oliver; Rees, Douglas C.

In: Biochemistry, Vol. 54, No. 11, 24.03.2015, p. 2052-2060.

Research output: Contribution to journalArticle

Morrison, Christine N. ; Hoy, Julie A. ; Zhang, Limei ; Einsle, Oliver ; Rees, Douglas C. / Substrate Pathways in the nitrogenase MoFe protein by experimental identification of small molecule binding sites. In: Biochemistry. 2015 ; Vol. 54, No. 11. pp. 2052-2060.
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