Substrate binding changes conformation of the α-, but not the β-subunit of mitochondrial processing peptidase

Oleksandr Gakh, Tomas Obsil, Jiri Adamec, Jaroslav Spizek, Evzen Amler, Jiri Janata, Frantisek Kalousek

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Lifetime analysis of tryptophan fluorescence of the mitochondrial processing peptidase (MPP) from Saccharomyces cerevisiae clearly proved that substrate binding evoked a conformational change of the α-subunit while presence of substrate influenced neither the lifetime components nor the average lifetime of the tryptophan excited state of the β-MPP subunit. Interestingly, lifetime analysis of tryptophan fluorescence decay of the α-MPP subunit revealed about 11% of steady-state fractional intensity due to the long-lived lifetime component, indicating that at least one tryptophan residue is partly buried at the hydrophobic microenvironment. Computer modeling, however, predicted none of three tryptophans, which the α-sub-unit contains, as deeply buried in the protein matrix. We conclude this as a consequence of a possible dimeric (oligomeric) structure.

Original languageEnglish (US)
Pages (from-to)392-396
Number of pages5
JournalArchives of Biochemistry and Biophysics
Volume385
Issue number2
DOIs
StatePublished - Jan 15 2001

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Tryptophan
Conformations
Substrates
Fluorescence
Excited states
Yeast
Saccharomyces cerevisiae
mitochondrial processing peptidase
Proteins

Keywords

  • Mitochondrial processing peptidase (MPP)
  • Structure
  • Tryptophan fluorescence
  • Yeast

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

Cite this

Substrate binding changes conformation of the α-, but not the β-subunit of mitochondrial processing peptidase. / Gakh, Oleksandr; Obsil, Tomas; Adamec, Jiri; Spizek, Jaroslav; Amler, Evzen; Janata, Jiri; Kalousek, Frantisek.

In: Archives of Biochemistry and Biophysics, Vol. 385, No. 2, 15.01.2001, p. 392-396.

Research output: Contribution to journalArticle

Gakh, Oleksandr ; Obsil, Tomas ; Adamec, Jiri ; Spizek, Jaroslav ; Amler, Evzen ; Janata, Jiri ; Kalousek, Frantisek. / Substrate binding changes conformation of the α-, but not the β-subunit of mitochondrial processing peptidase. In: Archives of Biochemistry and Biophysics. 2001 ; Vol. 385, No. 2. pp. 392-396.
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AU - Amler, Evzen

AU - Janata, Jiri

AU - Kalousek, Frantisek

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