Substance P increases neutrophil adhesion to bronchial epithelial cells

Virginia DeRose, Richard A. Robbins, R. Michael Snider, John R. Spurzem, Geoffrey M. Thiele, Stephen I. Rennard, Israel Rubinstein

Research output: Contribution to journalArticle

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Abstract

Polymorphonuclear neutrophils (PMNs) accumulate within the airways during acute and chronic bronchitis and can adhere to bronchial epithelium. Substance P (SP), a neuropeptide released from the primary afferent nerve endings, has been shown to have a proinflammatory effect on PMNs. To test the hypothesis that SP could modulate PMN bronchial epithelial cell adherence, bovine bronchial epithelial cells (BBECs) were isolated and cultured, and the capacity of SP to modulate PMN-BBEC adherence was evaluated. SP interacted with BBECs to induce an increase in PMN adhesion (14.7 ± 1.2% vs 5.3 ± 0.7% adherence, p < 0.01). The effect of SP was both time- and dose-dependent with maximal responses at 6 h and 10-10 M. The effect was reproduced by the carboxyl-terminal sequence of the molecule (SP 6-11). Importantly, pretreatment of the BBECs with the tachykinin SP receptor (NK1) antagonist, CP-96,345, significantly reduced the increase in adhesion induced by SP (p < 0.01). Furthermore, treatment of the BBECs with antibodies against CD11a (LFA-1), CD11b (MAC-1), or ICAM-1 significantly decreased SP-induced adherence (p < 0.01 all comparisons). Conversely, SP stimulation of PMN induced a dose-dependent, rapid (within 5 min) increase in adherence. This effect was also mediated by the carboxy end of the molecule and was decreased by CP-96,345, again suggesting that this effect was NK1 mediated. These data demonstrate the SP has the capacity for modulating PMN-BBEC interactions and suggest an important role for SP in modulating airway inflammation.

Original languageEnglish (US)
Pages (from-to)1339-1346
Number of pages8
JournalJournal of Immunology
Volume152
Issue number3
StatePublished - Feb 1 1994

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Substance P
Neutrophils
Epithelial Cells
Neurokinin-1 Receptor Antagonists
Tachykinins
Lymphocyte Function-Associated Antigen-1
Nerve Endings
Chronic Bronchitis
Intercellular Adhesion Molecule-1
Neuropeptides
Cell Communication
Epithelium
Inflammation
Antibodies

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

DeRose, V., Robbins, R. A., Snider, R. M., Spurzem, J. R., Thiele, G. M., Rennard, S. I., & Rubinstein, I. (1994). Substance P increases neutrophil adhesion to bronchial epithelial cells. Journal of Immunology, 152(3), 1339-1346.

Substance P increases neutrophil adhesion to bronchial epithelial cells. / DeRose, Virginia; Robbins, Richard A.; Snider, R. Michael; Spurzem, John R.; Thiele, Geoffrey M.; Rennard, Stephen I.; Rubinstein, Israel.

In: Journal of Immunology, Vol. 152, No. 3, 01.02.1994, p. 1339-1346.

Research output: Contribution to journalArticle

DeRose, V, Robbins, RA, Snider, RM, Spurzem, JR, Thiele, GM, Rennard, SI & Rubinstein, I 1994, 'Substance P increases neutrophil adhesion to bronchial epithelial cells', Journal of Immunology, vol. 152, no. 3, pp. 1339-1346.
DeRose V, Robbins RA, Snider RM, Spurzem JR, Thiele GM, Rennard SI et al. Substance P increases neutrophil adhesion to bronchial epithelial cells. Journal of Immunology. 1994 Feb 1;152(3):1339-1346.
DeRose, Virginia ; Robbins, Richard A. ; Snider, R. Michael ; Spurzem, John R. ; Thiele, Geoffrey M. ; Rennard, Stephen I. ; Rubinstein, Israel. / Substance P increases neutrophil adhesion to bronchial epithelial cells. In: Journal of Immunology. 1994 ; Vol. 152, No. 3. pp. 1339-1346.
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