Subcellular organization of calcium signalling in hepatocytes and the intact liver.

A. P. Thomas, D. C. Renard-Rooney, G. Hajnóczky, L. D. Robb-Gaspers, Chi Lin, T. A. Rooney

Research output: Contribution to journalReview article

26 Citations (Scopus)

Abstract

Hepatocytes respond to inositol 1,4,5-trisphosphate (InsP3)-linked agonists with frequency-modulated oscillations in the intracellular free calcium concentration ([Ca2+]i), that occur as waves propagating from a specific origin within each cell. The subcellular distribution and functional organization of InsP3-sensitive Ca2+ pools has been investigated, in both intact and permeabilized cells, by fluorescence imaging of dyes which can be used to monitor luminal Ca2+ content and InsP3-activated ion permeability in a spatially resolved manner. The Ca2+ stores behave as a luminally continuous system distributed throughout the cytoplasm. The structure of the stores, an important determinant of their function, is controlled by the cytoskeleton and can be modulated in a guanine nucleotide-dependent manner. The nuclear matrix is devoid of Ca2+ stores, but Ca2+ waves in the intact cell propagate through this compartment. The organization of [Ca2+]i signals has also been investigated in the perfused liver. Frequency-modulated [Ca2+]i oscillations are still observed at the single cell level, with similar properties to those in the isolated hepatocyte. The [Ca2+]i oscillations propagate between cells in the intact liver, leading to the synchronization of [Ca2+]i signals across part or all of each hepatic lobule.

Original languageEnglish (US)
JournalCiba Foundation symposium
Volume188
StatePublished - Jan 1 1995

Fingerprint

Calcium Signaling
Hepatocytes
Liver
Nuclear Matrix
Computer Communication Networks
Inositol 1,4,5-Trisphosphate
Guanine Nucleotides
Optical Imaging
Cytoskeleton
Permeability
Cytoplasm
Coloring Agents
Ions
Calcium

ASJC Scopus subject areas

  • General

Cite this

Thomas, A. P., Renard-Rooney, D. C., Hajnóczky, G., Robb-Gaspers, L. D., Lin, C., & Rooney, T. A. (1995). Subcellular organization of calcium signalling in hepatocytes and the intact liver. Ciba Foundation symposium, 188.

Subcellular organization of calcium signalling in hepatocytes and the intact liver. / Thomas, A. P.; Renard-Rooney, D. C.; Hajnóczky, G.; Robb-Gaspers, L. D.; Lin, Chi; Rooney, T. A.

In: Ciba Foundation symposium, Vol. 188, 01.01.1995.

Research output: Contribution to journalReview article

Thomas, AP, Renard-Rooney, DC, Hajnóczky, G, Robb-Gaspers, LD, Lin, C & Rooney, TA 1995, 'Subcellular organization of calcium signalling in hepatocytes and the intact liver.', Ciba Foundation symposium, vol. 188.
Thomas AP, Renard-Rooney DC, Hajnóczky G, Robb-Gaspers LD, Lin C, Rooney TA. Subcellular organization of calcium signalling in hepatocytes and the intact liver. Ciba Foundation symposium. 1995 Jan 1;188.
Thomas, A. P. ; Renard-Rooney, D. C. ; Hajnóczky, G. ; Robb-Gaspers, L. D. ; Lin, Chi ; Rooney, T. A. / Subcellular organization of calcium signalling in hepatocytes and the intact liver. In: Ciba Foundation symposium. 1995 ; Vol. 188.
@article{61c52475d86146d88845c0cfb73dd8c9,
title = "Subcellular organization of calcium signalling in hepatocytes and the intact liver.",
abstract = "Hepatocytes respond to inositol 1,4,5-trisphosphate (InsP3)-linked agonists with frequency-modulated oscillations in the intracellular free calcium concentration ([Ca2+]i), that occur as waves propagating from a specific origin within each cell. The subcellular distribution and functional organization of InsP3-sensitive Ca2+ pools has been investigated, in both intact and permeabilized cells, by fluorescence imaging of dyes which can be used to monitor luminal Ca2+ content and InsP3-activated ion permeability in a spatially resolved manner. The Ca2+ stores behave as a luminally continuous system distributed throughout the cytoplasm. The structure of the stores, an important determinant of their function, is controlled by the cytoskeleton and can be modulated in a guanine nucleotide-dependent manner. The nuclear matrix is devoid of Ca2+ stores, but Ca2+ waves in the intact cell propagate through this compartment. The organization of [Ca2+]i signals has also been investigated in the perfused liver. Frequency-modulated [Ca2+]i oscillations are still observed at the single cell level, with similar properties to those in the isolated hepatocyte. The [Ca2+]i oscillations propagate between cells in the intact liver, leading to the synchronization of [Ca2+]i signals across part or all of each hepatic lobule.",
author = "Thomas, {A. P.} and Renard-Rooney, {D. C.} and G. Hajn{\'o}czky and Robb-Gaspers, {L. D.} and Chi Lin and Rooney, {T. A.}",
year = "1995",
month = "1",
day = "1",
language = "English (US)",
volume = "188",
journal = "Ciba Foundation symposium",
issn = "0300-5208",
publisher = "Wiley Subscription Services",

}

TY - JOUR

T1 - Subcellular organization of calcium signalling in hepatocytes and the intact liver.

AU - Thomas, A. P.

AU - Renard-Rooney, D. C.

AU - Hajnóczky, G.

AU - Robb-Gaspers, L. D.

AU - Lin, Chi

AU - Rooney, T. A.

PY - 1995/1/1

Y1 - 1995/1/1

N2 - Hepatocytes respond to inositol 1,4,5-trisphosphate (InsP3)-linked agonists with frequency-modulated oscillations in the intracellular free calcium concentration ([Ca2+]i), that occur as waves propagating from a specific origin within each cell. The subcellular distribution and functional organization of InsP3-sensitive Ca2+ pools has been investigated, in both intact and permeabilized cells, by fluorescence imaging of dyes which can be used to monitor luminal Ca2+ content and InsP3-activated ion permeability in a spatially resolved manner. The Ca2+ stores behave as a luminally continuous system distributed throughout the cytoplasm. The structure of the stores, an important determinant of their function, is controlled by the cytoskeleton and can be modulated in a guanine nucleotide-dependent manner. The nuclear matrix is devoid of Ca2+ stores, but Ca2+ waves in the intact cell propagate through this compartment. The organization of [Ca2+]i signals has also been investigated in the perfused liver. Frequency-modulated [Ca2+]i oscillations are still observed at the single cell level, with similar properties to those in the isolated hepatocyte. The [Ca2+]i oscillations propagate between cells in the intact liver, leading to the synchronization of [Ca2+]i signals across part or all of each hepatic lobule.

AB - Hepatocytes respond to inositol 1,4,5-trisphosphate (InsP3)-linked agonists with frequency-modulated oscillations in the intracellular free calcium concentration ([Ca2+]i), that occur as waves propagating from a specific origin within each cell. The subcellular distribution and functional organization of InsP3-sensitive Ca2+ pools has been investigated, in both intact and permeabilized cells, by fluorescence imaging of dyes which can be used to monitor luminal Ca2+ content and InsP3-activated ion permeability in a spatially resolved manner. The Ca2+ stores behave as a luminally continuous system distributed throughout the cytoplasm. The structure of the stores, an important determinant of their function, is controlled by the cytoskeleton and can be modulated in a guanine nucleotide-dependent manner. The nuclear matrix is devoid of Ca2+ stores, but Ca2+ waves in the intact cell propagate through this compartment. The organization of [Ca2+]i signals has also been investigated in the perfused liver. Frequency-modulated [Ca2+]i oscillations are still observed at the single cell level, with similar properties to those in the isolated hepatocyte. The [Ca2+]i oscillations propagate between cells in the intact liver, leading to the synchronization of [Ca2+]i signals across part or all of each hepatic lobule.

UR - http://www.scopus.com/inward/record.url?scp=0029188095&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029188095&partnerID=8YFLogxK

M3 - Review article

VL - 188

JO - Ciba Foundation symposium

JF - Ciba Foundation symposium

SN - 0300-5208

ER -