Subcellular distribution of 5-HT1B and 5-HT7 receptors in the mouse suprachiasmatic nucleus

Michael A. Belenky, Gary E Pickard

Research output: Contribution to journalArticle

88 Citations (Scopus)

Abstract

The suprachiasmatic nucleus (SCN), a circadian oscillator, receives glutamatergic afferents from the retina and serotonergic (5-HT) afferents from the median raphe. 5-HT1B and 5-HT7 receptor agonists inhibit the effects of light on SCN circadian activity, Electron microscopic (EM) immunocytochemical procedures were used to determine the subcellular localization of 5-HT1B and 5-HT7 receptors in the SCN. 5-HT1B receptor immunostaining was associated with the plasma membrane of thin unmyelinated axons, preterminal axons, and terminals of optic and nonoptic origin, 5-HT1B receptor immunostaining in terminals was almost never observed at the synaptic active zone. To a much lesser extent, 5-HT1B immunoreaction product was noted in dendrites and somata of SCN neurons. 5-HT7 receptor immunoreactivity in γ-aminobutyric acid (GABA), vasoactive intestinal polypeptide (VIP), and vasopressin (VP) neuronal elements in the SCN was examined by using double-label procedures. 5-HT7 receptor immunoreaction product was often observed in GABA-, VIP-, and VP-immunoreactive dendrites as postsynaptic receptors and in axonal terminals as presynaptic receptors. 5-HT7 receptor immunoreactivity in terminals and dendrites was often associated with the plasma membrane but very seldom at the active zone. In GABA-, VIP-, and VP-immunoreactive perikarya, 5-HT7 receptor immunoreaction product was distributed throughout the cytoplasm often in association with the endoplasmic reticulum and the Golgi complex. The distribution of 5-HT1B receptors in presynaptic afferent terminals and postsynaptic SCN processes, as well as the distribution of 5-HT7 receptors in both pre- and postsynaptic GABA, VIP, and VP SCN processes, suggests that serotonin plays a significant role in the regulation of circadian rhythms by modulating SCN synaptic activity.

Original languageEnglish (US)
Pages (from-to)371-388
Number of pages18
JournalJournal of Comparative Neurology
Volume432
Issue number3
DOIs
StatePublished - Apr 9 2001

Fingerprint

Suprachiasmatic Nucleus
Vasoactive Intestinal Peptide
Receptor, Serotonin, 5-HT1B
Vasopressins
gamma-Aminobutyric Acid
Dendrites
Presynaptic Terminals
Serotonin
Cell Membrane
Aminobutyrates
Presynaptic Receptors
serotonin 7 receptor
Golgi Apparatus
Carisoprodol
Circadian Rhythm
Endoplasmic Reticulum
Axons
Retina
Cytoplasm
Electrons

Keywords

  • 5-HT receptor subtypes
  • Circadian rhythms
  • Intergeniculate leaflet
  • Retinal ganglion cells
  • Retinohypothalamic tract
  • Serotonin

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Subcellular distribution of 5-HT1B and 5-HT7 receptors in the mouse suprachiasmatic nucleus. / Belenky, Michael A.; Pickard, Gary E.

In: Journal of Comparative Neurology, Vol. 432, No. 3, 09.04.2001, p. 371-388.

Research output: Contribution to journalArticle

@article{738f83980e92466ca5998cf8d84a70c3,
title = "Subcellular distribution of 5-HT1B and 5-HT7 receptors in the mouse suprachiasmatic nucleus",
abstract = "The suprachiasmatic nucleus (SCN), a circadian oscillator, receives glutamatergic afferents from the retina and serotonergic (5-HT) afferents from the median raphe. 5-HT1B and 5-HT7 receptor agonists inhibit the effects of light on SCN circadian activity, Electron microscopic (EM) immunocytochemical procedures were used to determine the subcellular localization of 5-HT1B and 5-HT7 receptors in the SCN. 5-HT1B receptor immunostaining was associated with the plasma membrane of thin unmyelinated axons, preterminal axons, and terminals of optic and nonoptic origin, 5-HT1B receptor immunostaining in terminals was almost never observed at the synaptic active zone. To a much lesser extent, 5-HT1B immunoreaction product was noted in dendrites and somata of SCN neurons. 5-HT7 receptor immunoreactivity in γ-aminobutyric acid (GABA), vasoactive intestinal polypeptide (VIP), and vasopressin (VP) neuronal elements in the SCN was examined by using double-label procedures. 5-HT7 receptor immunoreaction product was often observed in GABA-, VIP-, and VP-immunoreactive dendrites as postsynaptic receptors and in axonal terminals as presynaptic receptors. 5-HT7 receptor immunoreactivity in terminals and dendrites was often associated with the plasma membrane but very seldom at the active zone. In GABA-, VIP-, and VP-immunoreactive perikarya, 5-HT7 receptor immunoreaction product was distributed throughout the cytoplasm often in association with the endoplasmic reticulum and the Golgi complex. The distribution of 5-HT1B receptors in presynaptic afferent terminals and postsynaptic SCN processes, as well as the distribution of 5-HT7 receptors in both pre- and postsynaptic GABA, VIP, and VP SCN processes, suggests that serotonin plays a significant role in the regulation of circadian rhythms by modulating SCN synaptic activity.",
keywords = "5-HT receptor subtypes, Circadian rhythms, Intergeniculate leaflet, Retinal ganglion cells, Retinohypothalamic tract, Serotonin",
author = "Belenky, {Michael A.} and Pickard, {Gary E}",
year = "2001",
month = "4",
day = "9",
doi = "10.1002/cne.1109",
language = "English (US)",
volume = "432",
pages = "371--388",
journal = "Journal of Comparative Neurology",
issn = "0021-9967",
publisher = "Wiley-Liss Inc.",
number = "3",

}

TY - JOUR

T1 - Subcellular distribution of 5-HT1B and 5-HT7 receptors in the mouse suprachiasmatic nucleus

AU - Belenky, Michael A.

AU - Pickard, Gary E

PY - 2001/4/9

Y1 - 2001/4/9

N2 - The suprachiasmatic nucleus (SCN), a circadian oscillator, receives glutamatergic afferents from the retina and serotonergic (5-HT) afferents from the median raphe. 5-HT1B and 5-HT7 receptor agonists inhibit the effects of light on SCN circadian activity, Electron microscopic (EM) immunocytochemical procedures were used to determine the subcellular localization of 5-HT1B and 5-HT7 receptors in the SCN. 5-HT1B receptor immunostaining was associated with the plasma membrane of thin unmyelinated axons, preterminal axons, and terminals of optic and nonoptic origin, 5-HT1B receptor immunostaining in terminals was almost never observed at the synaptic active zone. To a much lesser extent, 5-HT1B immunoreaction product was noted in dendrites and somata of SCN neurons. 5-HT7 receptor immunoreactivity in γ-aminobutyric acid (GABA), vasoactive intestinal polypeptide (VIP), and vasopressin (VP) neuronal elements in the SCN was examined by using double-label procedures. 5-HT7 receptor immunoreaction product was often observed in GABA-, VIP-, and VP-immunoreactive dendrites as postsynaptic receptors and in axonal terminals as presynaptic receptors. 5-HT7 receptor immunoreactivity in terminals and dendrites was often associated with the plasma membrane but very seldom at the active zone. In GABA-, VIP-, and VP-immunoreactive perikarya, 5-HT7 receptor immunoreaction product was distributed throughout the cytoplasm often in association with the endoplasmic reticulum and the Golgi complex. The distribution of 5-HT1B receptors in presynaptic afferent terminals and postsynaptic SCN processes, as well as the distribution of 5-HT7 receptors in both pre- and postsynaptic GABA, VIP, and VP SCN processes, suggests that serotonin plays a significant role in the regulation of circadian rhythms by modulating SCN synaptic activity.

AB - The suprachiasmatic nucleus (SCN), a circadian oscillator, receives glutamatergic afferents from the retina and serotonergic (5-HT) afferents from the median raphe. 5-HT1B and 5-HT7 receptor agonists inhibit the effects of light on SCN circadian activity, Electron microscopic (EM) immunocytochemical procedures were used to determine the subcellular localization of 5-HT1B and 5-HT7 receptors in the SCN. 5-HT1B receptor immunostaining was associated with the plasma membrane of thin unmyelinated axons, preterminal axons, and terminals of optic and nonoptic origin, 5-HT1B receptor immunostaining in terminals was almost never observed at the synaptic active zone. To a much lesser extent, 5-HT1B immunoreaction product was noted in dendrites and somata of SCN neurons. 5-HT7 receptor immunoreactivity in γ-aminobutyric acid (GABA), vasoactive intestinal polypeptide (VIP), and vasopressin (VP) neuronal elements in the SCN was examined by using double-label procedures. 5-HT7 receptor immunoreaction product was often observed in GABA-, VIP-, and VP-immunoreactive dendrites as postsynaptic receptors and in axonal terminals as presynaptic receptors. 5-HT7 receptor immunoreactivity in terminals and dendrites was often associated with the plasma membrane but very seldom at the active zone. In GABA-, VIP-, and VP-immunoreactive perikarya, 5-HT7 receptor immunoreaction product was distributed throughout the cytoplasm often in association with the endoplasmic reticulum and the Golgi complex. The distribution of 5-HT1B receptors in presynaptic afferent terminals and postsynaptic SCN processes, as well as the distribution of 5-HT7 receptors in both pre- and postsynaptic GABA, VIP, and VP SCN processes, suggests that serotonin plays a significant role in the regulation of circadian rhythms by modulating SCN synaptic activity.

KW - 5-HT receptor subtypes

KW - Circadian rhythms

KW - Intergeniculate leaflet

KW - Retinal ganglion cells

KW - Retinohypothalamic tract

KW - Serotonin

UR - http://www.scopus.com/inward/record.url?scp=0035832118&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035832118&partnerID=8YFLogxK

U2 - 10.1002/cne.1109

DO - 10.1002/cne.1109

M3 - Article

VL - 432

SP - 371

EP - 388

JO - Journal of Comparative Neurology

JF - Journal of Comparative Neurology

SN - 0021-9967

IS - 3

ER -