Studies of the CobA-type ATP:Co(I)rrinoid adenosyltransferase enzyme of Methanosarcina mazei strain Gö1

Nicole R. Buan, Kimberly Rehfeld, Jorge C. Escalante-Semerena

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Although methanogenic archaea use B12 extensively as a methyl carrier for methanogenesis, little is known about B12 metabolism in these prokaryotes or any other archaea. To improve our understanding of how B12 metabolism differs between bacteria and archaea, the gene encoding the ATP:co(I)rrinoid adenosyltransferase in Methanosarcina mazei strain Gö1 (open reading frame MM3138, referred to as cobAMm here) was cloned and used to restore coenzyme B12 synthesis in a Salmonella enterica strain lacking the housekeeping CobA enzyme. cobAMm protein was purified and its initial biochemical analysis performed. In vitro, the activity is enhanced 2.5-fold by the addition of Ca2+ ions, but the activity was not enhanced by Mg2+ and, unlike the 5. enterica CobA enzyme, it was >50% inhibited by Mn2+. The CobAMm enzyme had a KmATP of 3 μM and a KmHOCb1 of 1 μM. Unlike the S. enterica enzyme, CobAMm used cobalamin (Cb1) as a substrate better than cobinamide (Cbi; a Cbl precursor); the β phosphate of ATP was required for binding to the enzyme. A striking difference between CobASe and CobAMm was the use of ADP as a substrate by CobAMm, suggesting an important role for the γ phosphate of ATP in binding. The results from 31P-nuclear magnetic resonance spectroscopy experiments showed that triphosphate (PPPi) is the reaction by-product; no cleavage of PPPi was observed, and the enzyme was only slightly inhibited by pyrophosphate (PPi). The data suggested substantial variations in ATP binding and probably corrinoid binding between CobASe and CobAMm enzymes.

Original languageEnglish (US)
Pages (from-to)3543-3550
Number of pages8
JournalJournal of bacteriology
Volume188
Issue number10
DOIs
StatePublished - May 1 2006

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Methanosarcina
Enzymes
Archaea
Salmonella enterica
Adenosine Triphosphate
Corrinoids
Phosphates
Housekeeping
Vitamin B 12
ATP-corrinoid adenosyltransferase
Adenosine Diphosphate
Open Reading Frames
Magnetic Resonance Spectroscopy
Ions
Bacteria

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

Studies of the CobA-type ATP:Co(I)rrinoid adenosyltransferase enzyme of Methanosarcina mazei strain Gö1. / Buan, Nicole R.; Rehfeld, Kimberly; Escalante-Semerena, Jorge C.

In: Journal of bacteriology, Vol. 188, No. 10, 01.05.2006, p. 3543-3550.

Research output: Contribution to journalArticle

Buan, Nicole R. ; Rehfeld, Kimberly ; Escalante-Semerena, Jorge C. / Studies of the CobA-type ATP:Co(I)rrinoid adenosyltransferase enzyme of Methanosarcina mazei strain Gö1. In: Journal of bacteriology. 2006 ; Vol. 188, No. 10. pp. 3543-3550.
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abstract = "Although methanogenic archaea use B12 extensively as a methyl carrier for methanogenesis, little is known about B12 metabolism in these prokaryotes or any other archaea. To improve our understanding of how B12 metabolism differs between bacteria and archaea, the gene encoding the ATP:co(I)rrinoid adenosyltransferase in Methanosarcina mazei strain G{\"o}1 (open reading frame MM3138, referred to as cobAMm here) was cloned and used to restore coenzyme B12 synthesis in a Salmonella enterica strain lacking the housekeeping CobA enzyme. cobAMm protein was purified and its initial biochemical analysis performed. In vitro, the activity is enhanced 2.5-fold by the addition of Ca2+ ions, but the activity was not enhanced by Mg2+ and, unlike the 5. enterica CobA enzyme, it was >50{\%} inhibited by Mn2+. The CobAMm enzyme had a KmATP of 3 μM and a KmHOCb1 of 1 μM. Unlike the S. enterica enzyme, CobAMm used cobalamin (Cb1) as a substrate better than cobinamide (Cbi; a Cbl precursor); the β phosphate of ATP was required for binding to the enzyme. A striking difference between CobASe and CobAMm was the use of ADP as a substrate by CobAMm, suggesting an important role for the γ phosphate of ATP in binding. The results from 31P-nuclear magnetic resonance spectroscopy experiments showed that triphosphate (PPPi) is the reaction by-product; no cleavage of PPPi was observed, and the enzyme was only slightly inhibited by pyrophosphate (PPi). The data suggested substantial variations in ATP binding and probably corrinoid binding between CobASe and CobAMm enzymes.",
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