Studies of antibody-antigen interactions by capillary electrophoresis

A review

Annette C Moser, Sidney Trenhaile, Kati Frankenberg

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Antibody-antigen interactions are vital in immunoassay development and can determine detection limits and analysis times. Capillary electrophoresis (CE) is a powerful technique that can be used to quantify antibody-antigen interactions. These CE methods range from simple separations of a premixed antibody and antigen sample applied as a short plug to allow for separation of complex, free antibody, and free antigen to more complex systems which inject complexed samples in the presence of antibody or antigen; or even injections of antibody and antigen sequentially. The objective of this review is to identify and describe various CE techniques which have been used to study antibody-antigen interactions. A brief discussion of linear and nonlinear curve fitting is also included.

Original languageEnglish (US)
JournalMethods
DOIs
StateAccepted/In press - Jan 1 2018

Fingerprint

Capillary electrophoresis
Capillary Electrophoresis
Antigens
Antibodies
Curve fitting
Immunoassay
Limit of Detection
Large scale systems
Injections

Keywords

  • Affinity capillary electrophoresis
  • Antibody-antigen interactions
  • Capillary electrophoresis frontal analysis
  • Flow through partial filling affinity capillary electrophoresis
  • Multiple sequential injections of equal volumes
  • Partial filling affinity capillary electrophoresis

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Studies of antibody-antigen interactions by capillary electrophoresis : A review. / Moser, Annette C; Trenhaile, Sidney; Frankenberg, Kati.

In: Methods, 01.01.2018.

Research output: Contribution to journalArticle

@article{5111fbd745d9440c98819839dda2eb9a,
title = "Studies of antibody-antigen interactions by capillary electrophoresis: A review",
abstract = "Antibody-antigen interactions are vital in immunoassay development and can determine detection limits and analysis times. Capillary electrophoresis (CE) is a powerful technique that can be used to quantify antibody-antigen interactions. These CE methods range from simple separations of a premixed antibody and antigen sample applied as a short plug to allow for separation of complex, free antibody, and free antigen to more complex systems which inject complexed samples in the presence of antibody or antigen; or even injections of antibody and antigen sequentially. The objective of this review is to identify and describe various CE techniques which have been used to study antibody-antigen interactions. A brief discussion of linear and nonlinear curve fitting is also included.",
keywords = "Affinity capillary electrophoresis, Antibody-antigen interactions, Capillary electrophoresis frontal analysis, Flow through partial filling affinity capillary electrophoresis, Multiple sequential injections of equal volumes, Partial filling affinity capillary electrophoresis",
author = "Moser, {Annette C} and Sidney Trenhaile and Kati Frankenberg",
year = "2018",
month = "1",
day = "1",
doi = "10.1016/j.ymeth.2018.03.006",
language = "English (US)",
journal = "ImmunoMethods",
issn = "1046-2023",
publisher = "Academic Press Inc.",

}

TY - JOUR

T1 - Studies of antibody-antigen interactions by capillary electrophoresis

T2 - A review

AU - Moser, Annette C

AU - Trenhaile, Sidney

AU - Frankenberg, Kati

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Antibody-antigen interactions are vital in immunoassay development and can determine detection limits and analysis times. Capillary electrophoresis (CE) is a powerful technique that can be used to quantify antibody-antigen interactions. These CE methods range from simple separations of a premixed antibody and antigen sample applied as a short plug to allow for separation of complex, free antibody, and free antigen to more complex systems which inject complexed samples in the presence of antibody or antigen; or even injections of antibody and antigen sequentially. The objective of this review is to identify and describe various CE techniques which have been used to study antibody-antigen interactions. A brief discussion of linear and nonlinear curve fitting is also included.

AB - Antibody-antigen interactions are vital in immunoassay development and can determine detection limits and analysis times. Capillary electrophoresis (CE) is a powerful technique that can be used to quantify antibody-antigen interactions. These CE methods range from simple separations of a premixed antibody and antigen sample applied as a short plug to allow for separation of complex, free antibody, and free antigen to more complex systems which inject complexed samples in the presence of antibody or antigen; or even injections of antibody and antigen sequentially. The objective of this review is to identify and describe various CE techniques which have been used to study antibody-antigen interactions. A brief discussion of linear and nonlinear curve fitting is also included.

KW - Affinity capillary electrophoresis

KW - Antibody-antigen interactions

KW - Capillary electrophoresis frontal analysis

KW - Flow through partial filling affinity capillary electrophoresis

KW - Multiple sequential injections of equal volumes

KW - Partial filling affinity capillary electrophoresis

UR - http://www.scopus.com/inward/record.url?scp=85044070522&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85044070522&partnerID=8YFLogxK

U2 - 10.1016/j.ymeth.2018.03.006

DO - 10.1016/j.ymeth.2018.03.006

M3 - Article

JO - ImmunoMethods

JF - ImmunoMethods

SN - 1046-2023

ER -