Studies of antibody-antigen interactions by capillary electrophoresis: A review

Annette C. Moser, Sidney Trenhaile, Kati Frankenberg

Research output: Contribution to journalReview article

5 Citations (Scopus)

Abstract

Antibody-antigen interactions are vital in immunoassay development and can determine detection limits and analysis times. Capillary electrophoresis (CE) is a powerful technique that can be used to quantify antibody-antigen interactions. These CE methods range from simple separations of a premixed antibody and antigen sample applied as a short plug to allow for separation of complex, free antibody, and free antigen to more complex systems which inject complexed samples in the presence of antibody or antigen; or even injections of antibody and antigen sequentially. The objective of this review is to identify and describe various CE techniques which have been used to study antibody-antigen interactions. A brief discussion of linear and nonlinear curve fitting is also included.

Original languageEnglish (US)
Pages (from-to)66-75
Number of pages10
JournalMethods
Volume146
DOIs
StatePublished - Aug 15 2018

Fingerprint

Capillary electrophoresis
Capillary Electrophoresis
Antigens
Antibodies
Curve fitting
Immunoassay
Limit of Detection
Large scale systems
Injections

Keywords

  • Affinity capillary electrophoresis
  • Antibody-antigen interactions
  • Capillary electrophoresis frontal analysis
  • Flow through partial filling affinity capillary electrophoresis
  • Multiple sequential injections of equal volumes
  • Partial filling affinity capillary electrophoresis

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Studies of antibody-antigen interactions by capillary electrophoresis : A review. / Moser, Annette C.; Trenhaile, Sidney; Frankenberg, Kati.

In: Methods, Vol. 146, 15.08.2018, p. 66-75.

Research output: Contribution to journalReview article

Moser, Annette C. ; Trenhaile, Sidney ; Frankenberg, Kati. / Studies of antibody-antigen interactions by capillary electrophoresis : A review. In: Methods. 2018 ; Vol. 146. pp. 66-75.
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