Structure of cytochrome c' from Rhodobacter capsulatus strain St Louis

An unusual molecular association induced by bridging Zn ions

Tahir H Tahirov, Shintaro Misaki, Terry E. Meyer, Michael A. Cusanovich, Yoshiki Higuchi, Noritake Yasuoka

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Rhodobacter capsulatus strain St Louis cytochrome c' (RCCP-SL) has been crystallized and the structure solved by molecular replacement. It was refined at 2.1 Å resolution to an R value of 18.4%, and compared with Rhodobacter capsulatus strain M110 cytochrome c' (RCCP-M110). Although these two proteins are very similar in sequence and structure, the intermolecular interaction is largely different. In RCCP-M110, the molecules dimerize through interaction of helix B to form an antiparallel arrangement. When crystallized in the presence of Zn ions, molecules of RCCP-SL were found to be arranged as linear polymers connected by the bridging Zn ions. The changes in conformation of the side chains induced by binding of the Zn ions, by the substitution of Glu90 for Asp90, and by the different arrangement of the molecules, are discussed in detail.

Original languageEnglish (US)
Pages (from-to)658-664
Number of pages7
JournalActa Crystallographica Section D: Biological Crystallography
Volume53
Issue number6
DOIs
StatePublished - Jan 1 1997

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Cytochromes c'
Rhodobacter capsulatus
cytochromes
Association reactions
Ions
Molecules
molecules
ions
Molecular Structure
helices
Conformations
Polymers
Substitution reactions
interactions
substitutes
proteins
polymers
Proteins

ASJC Scopus subject areas

  • Structural Biology

Cite this

Structure of cytochrome c' from Rhodobacter capsulatus strain St Louis : An unusual molecular association induced by bridging Zn ions. / Tahirov, Tahir H; Misaki, Shintaro; Meyer, Terry E.; Cusanovich, Michael A.; Higuchi, Yoshiki; Yasuoka, Noritake.

In: Acta Crystallographica Section D: Biological Crystallography, Vol. 53, No. 6, 01.01.1997, p. 658-664.

Research output: Contribution to journalArticle

Tahirov, Tahir H ; Misaki, Shintaro ; Meyer, Terry E. ; Cusanovich, Michael A. ; Higuchi, Yoshiki ; Yasuoka, Noritake. / Structure of cytochrome c' from Rhodobacter capsulatus strain St Louis : An unusual molecular association induced by bridging Zn ions. In: Acta Crystallographica Section D: Biological Crystallography. 1997 ; Vol. 53, No. 6. pp. 658-664.
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