Structure of B-DNA with cations tethered in the major groove

Tinoush Moulaei, Tatsuya Maehigashi, George T. Lountos, Seiji Komeda, Derrick Watkins, Michael P. Stone, Luis A Marky, Jian Sen Li, Barry Gold, Loren Dean Williams

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Here, we describe the 1.6-Å X-ray structure of the DDD (Dickerson-Drew dodecamer), which has been covalently modified by the tethering of four cationic charges. This modified version of the DDD, called here the DDD4+, is composed of [d(CGCGAAXXCGCG)]2, where X is effectively a thymine residue linked at the 5 position to an n-propyl-amine. The structure was determined from crystals soaked with thallium(I), which has been broadly used as a mimic of K+ in X-ray diffraction experiments aimed at determining positions of cations adjacent to nucleic acids. Three of the tethered cations are directed radially out from the DNA. The radially directed tethered cations do not appear to induce structural changes or to displace counterions. One of the tethered cations is directed in the 3′ direction, toward a phosphate group near one end of the duplex. This tethered cation appears to interact electrostatically with the DNA. This interaction is accompanied by changes in helical parameters rise, roll, and twist and by a displacement of the backbone relative to a control oligonucleotide. In addition, these interactions appear to be associated with displacement of counterions from the major groove of the DNA.

Original languageEnglish (US)
Pages (from-to)7458-7468
Number of pages11
JournalBiochemistry
Volume44
Issue number20
DOIs
StatePublished - May 24 2005

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B-Form DNA
Cations
DNA
Thymine
Thallium
Oligonucleotides
X-Ray Diffraction
Nucleic Acids
Amines
Phosphates
X-Rays
X ray diffraction
X rays
Crystals
Experiments

ASJC Scopus subject areas

  • Biochemistry

Cite this

Moulaei, T., Maehigashi, T., Lountos, G. T., Komeda, S., Watkins, D., Stone, M. P., ... Williams, L. D. (2005). Structure of B-DNA with cations tethered in the major groove. Biochemistry, 44(20), 7458-7468. https://doi.org/10.1021/bi050128z

Structure of B-DNA with cations tethered in the major groove. / Moulaei, Tinoush; Maehigashi, Tatsuya; Lountos, George T.; Komeda, Seiji; Watkins, Derrick; Stone, Michael P.; Marky, Luis A; Li, Jian Sen; Gold, Barry; Williams, Loren Dean.

In: Biochemistry, Vol. 44, No. 20, 24.05.2005, p. 7458-7468.

Research output: Contribution to journalArticle

Moulaei, T, Maehigashi, T, Lountos, GT, Komeda, S, Watkins, D, Stone, MP, Marky, LA, Li, JS, Gold, B & Williams, LD 2005, 'Structure of B-DNA with cations tethered in the major groove', Biochemistry, vol. 44, no. 20, pp. 7458-7468. https://doi.org/10.1021/bi050128z
Moulaei T, Maehigashi T, Lountos GT, Komeda S, Watkins D, Stone MP et al. Structure of B-DNA with cations tethered in the major groove. Biochemistry. 2005 May 24;44(20):7458-7468. https://doi.org/10.1021/bi050128z
Moulaei, Tinoush ; Maehigashi, Tatsuya ; Lountos, George T. ; Komeda, Seiji ; Watkins, Derrick ; Stone, Michael P. ; Marky, Luis A ; Li, Jian Sen ; Gold, Barry ; Williams, Loren Dean. / Structure of B-DNA with cations tethered in the major groove. In: Biochemistry. 2005 ; Vol. 44, No. 20. pp. 7458-7468.
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