Structure of a closed-form uroporphyrinogen-III C-methyltransferase from Thermus thermophilus

Peter H. Rehse, Tomoe Kitao, Tahir H Tahirov

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Uroporphyrinogen-III C-methyltransferase from Thermus thermophilus is a multifunctional protein responsible for two of the eight S-adenosyl-methionine- dependent methylations of the corrin ring during vitamin B12 synthesis. The structure of this protein has been solved to 2.0 Å resolution in both the apo and cofactor-bound form. The monomer consists of two domains, A and B, each consisting of a five-stranded β-sheet and two or three α-helices, with the cofactor bound at the interface. The biological unit is the dimer found in the asymmetric unit. This dimer is related by a non-crystallographic twofold such that two B domains combine to form a long ten-stranded β-sheet. When compared with solved related structures, this structure shows clear differences in the region involved in cofactor and substrate binding, affirming the role of several previously implicated residues and questioning others. The solved related structures are characterized by an exposed active site. The T. thermophilus structure has this site restricted by the interaction of a flexible loop structure with a highly conserved residue, suggesting a mechanistic role. This structure represents the 'closed' form of the protein.

Original languageEnglish (US)
Pages (from-to)913-919
Number of pages7
JournalActa Crystallographica Section D: Biological Crystallography
Volume61
Issue number7
DOIs
StatePublished - Jul 1 2005

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Uroporphyrinogens
Thermus thermophilus
Methyltransferases
proteins
Dimers
dimers
vitamins
methionine
Proteins
methylation
Methylation
Vitamin B 12
Methionine
helices
Catalytic Domain
monomers
Monomers
rings
Substrates
synthesis

ASJC Scopus subject areas

  • Structural Biology
  • Medicine(all)

Cite this

Structure of a closed-form uroporphyrinogen-III C-methyltransferase from Thermus thermophilus. / Rehse, Peter H.; Kitao, Tomoe; Tahirov, Tahir H.

In: Acta Crystallographica Section D: Biological Crystallography, Vol. 61, No. 7, 01.07.2005, p. 913-919.

Research output: Contribution to journalArticle

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