Structure function analysis of an ADP-ribosyltransferase type III effector and its RNA-binding target in plant immunity

Byeong Ryool Jeong, Yan Lin, Anna Joe, Ming Guo, Christin Korneli, Huirong Yang, Ping Wang, Min Yu, Ronald Cerny, Dorothee Staiger, James R Alfano, Yanhui Xu

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

The Pseudomonas syringae type III effector HopU1 is a mono-ADP- ribosyltransferase that is injected into plant cells by the type III protein secretion system. Inside the plant cell it suppresses immunity by modifying RNA-binding proteins including the glycine-rich RNA-binding protein GRP7. The crystal structure of HopU1 at 2.7-Å resolution reveals two unique protruding loops, L1 and L4, not found in other mono-ADP-ribosyltransferases. Site-directed mutagenesis demonstrates that these loops are essential for substrate recognition and enzymatic activity. HopU1 ADP-ribosylates the conserved arginine 49 of GRP7, and this reduces the ability of GRP7 to bind RNA in vitro. In vivo, expression of GRP7 with Arg-49 replaced with lysine does not complement the reduced immune responses of the Arabidopsis thaliana grp7-1 mutant demonstrating the importance of this residue for GRP7 function. These data provide mechanistic details how HopU1 recognizes this novel type of substrate and highlights the role of GRP7 in plant immunity.

Original languageEnglish (US)
Pages (from-to)43272-43281
Number of pages10
JournalJournal of Biological Chemistry
Volume286
Issue number50
DOIs
StatePublished - Dec 16 2011

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Plant Immunity
ADP Ribose Transferases
RNA-Binding Proteins
Plant Cells
RNA
Pseudomonas syringae
Mutagenesis
Substrates
Site-Directed Mutagenesis
Arabidopsis
Glycine
Adenosine Diphosphate
Lysine
Arginine
Immunity
Crystal structure

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Structure function analysis of an ADP-ribosyltransferase type III effector and its RNA-binding target in plant immunity. / Jeong, Byeong Ryool; Lin, Yan; Joe, Anna; Guo, Ming; Korneli, Christin; Yang, Huirong; Wang, Ping; Yu, Min; Cerny, Ronald; Staiger, Dorothee; Alfano, James R; Xu, Yanhui.

In: Journal of Biological Chemistry, Vol. 286, No. 50, 16.12.2011, p. 43272-43281.

Research output: Contribution to journalArticle

Jeong, Byeong Ryool ; Lin, Yan ; Joe, Anna ; Guo, Ming ; Korneli, Christin ; Yang, Huirong ; Wang, Ping ; Yu, Min ; Cerny, Ronald ; Staiger, Dorothee ; Alfano, James R ; Xu, Yanhui. / Structure function analysis of an ADP-ribosyltransferase type III effector and its RNA-binding target in plant immunity. In: Journal of Biological Chemistry. 2011 ; Vol. 286, No. 50. pp. 43272-43281.
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