Structural studies demonstrating a bacteriophage-like replication cycle of the eukaryote-infecting Paramecium bursaria chlorella virus-1

Elad Milrot, Eyal Shimoni, Tali Dadosh, Katya Rechav, Tamar Unger, James L Van Etten, Abraham Minsky

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

A fundamental stage in viral infection is the internalization of viral genomes in host cells. Although extensively studied, the mechanisms and factors responsible for the genome internalization process remain poorly understood. Here we report our observations, derived from diverse imaging methods on genome internalization of the large dsDNA Paramecium bursaria chlorella virus-1 (PBCV-1). Our studies reveal that early infection stages of this eukaryotic-infecting virus occurs by a bacteriophage-like pathway, whereby PBCV-1 generates a hole in the host cell wall and ejects its dsDNA genome in a linear, base-pair-by-base-pair process, through a membrane tunnel generated by the fusion of the virus internal membrane with the host membrane. Furthermore, our results imply that PBCV-1 DNA condensation that occurs shortly after infection probably plays a role in genome internalization, as hypothesized for the infection of some bacteriophages. The subsequent perforation of the host photosynthetic membranes presumably enables trafficking of viral genomes towards host nuclei. Previous studies established that at late infection stages PBCV-1 generates cytoplasmic organelles, termed viral factories, where viral assembly takes place, a feature characteristic of many large dsDNA viruses that infect eukaryotic organisms. PBCV-1 thus appears to combine a bacteriophage-like mechanism during early infection stages with a eukaryotic-like infection pathway in its late replication cycle.

Original languageEnglish (US)
Article numbere1006562
JournalPLoS Pathogens
Volume13
Issue number8
DOIs
StatePublished - Aug 1 2017

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Paramecium
Chlorella
Eukaryota
Bacteriophages
Viruses
Infection
Genome
Viral Genome
Base Pairing
Membranes
Virus Assembly
Virus Internalization
DNA Viruses
Virus Diseases
Organelles
Cell Wall

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Molecular Biology
  • Genetics
  • Virology

Cite this

Structural studies demonstrating a bacteriophage-like replication cycle of the eukaryote-infecting Paramecium bursaria chlorella virus-1. / Milrot, Elad; Shimoni, Eyal; Dadosh, Tali; Rechav, Katya; Unger, Tamar; Van Etten, James L; Minsky, Abraham.

In: PLoS Pathogens, Vol. 13, No. 8, e1006562, 01.08.2017.

Research output: Contribution to journalArticle

Milrot, Elad ; Shimoni, Eyal ; Dadosh, Tali ; Rechav, Katya ; Unger, Tamar ; Van Etten, James L ; Minsky, Abraham. / Structural studies demonstrating a bacteriophage-like replication cycle of the eukaryote-infecting Paramecium bursaria chlorella virus-1. In: PLoS Pathogens. 2017 ; Vol. 13, No. 8.
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