Structural proteins and lipids in a virus, PBCV-1, which replicates in a chlorella-like alga

Merri P. Skrdla, Dwight E. Burbank, Yuannan Xia, Russel H. Meints, James L Van Etten

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

PBCV-1, a large dsDNA-containing virus which replicates in a Chlorella-like green alga, is composed of approximately 64% protein, 25% DNA, and 5-10% lipid on a weight basis. Polyacrylamide gel electrophoresis of the dissociated virus particle resolves 50 to 60 proteins which range in apparent molecular weight from 10,000 to 135,000. Two of these proteins are glycoproteins and at least four are located on the viral surface. The major lipids are phosphatidyl choline, phosphatidyl ethanolamine, and an unidentified component. The effect of organic solvents, surfactants, and chelating and reducing agents on viral infectivity and ultrastructure are reported. Inhibitor studies established that PBCV-1 protein synthesis occurs on cytoplasmic ribosomes.

Original languageEnglish (US)
Pages (from-to)308-315
Number of pages8
JournalVirology
Volume135
Issue number2
DOIs
StatePublished - Jan 1 1984

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Chlorella
Viruses
Lipids
Proteins
Chlorophyta
Ethanolamine
Reducing Agents
Chelating Agents
Phosphatidylcholines
Ribosomes
Surface-Active Agents
Virion
Polyacrylamide Gel Electrophoresis
Glycoproteins
Molecular Weight
Weights and Measures
DNA

ASJC Scopus subject areas

  • Virology

Cite this

Structural proteins and lipids in a virus, PBCV-1, which replicates in a chlorella-like alga. / Skrdla, Merri P.; Burbank, Dwight E.; Xia, Yuannan; Meints, Russel H.; Van Etten, James L.

In: Virology, Vol. 135, No. 2, 01.01.1984, p. 308-315.

Research output: Contribution to journalArticle

Skrdla, Merri P. ; Burbank, Dwight E. ; Xia, Yuannan ; Meints, Russel H. ; Van Etten, James L. / Structural proteins and lipids in a virus, PBCV-1, which replicates in a chlorella-like alga. In: Virology. 1984 ; Vol. 135, No. 2. pp. 308-315.
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