Structural proteins and lipids in a virus, PBCV-1, which replicates in a chlorella-like alga

Merri P. Skrdla, Dwight E. Burbank, Yuannan Xia, Russel H. Meints, James L. van Etten

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Abstract

PBCV-1, a large dsDNA-containing virus which replicates in a Chlorella-like green alga, is composed of approximately 64% protein, 25% DNA, and 5-10% lipid on a weight basis. Polyacrylamide gel electrophoresis of the dissociated virus particle resolves 50 to 60 proteins which range in apparent molecular weight from 10,000 to 135,000. Two of these proteins are glycoproteins and at least four are located on the viral surface. The major lipids are phosphatidyl choline, phosphatidyl ethanolamine, and an unidentified component. The effect of organic solvents, surfactants, and chelating and reducing agents on viral infectivity and ultrastructure are reported. Inhibitor studies established that PBCV-1 protein synthesis occurs on cytoplasmic ribosomes.

Original languageEnglish (US)
Pages (from-to)308-315
Number of pages8
JournalVirology
Volume135
Issue number2
DOIs
StatePublished - Jun 1984

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ASJC Scopus subject areas

  • Virology

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