Structural organization of DNA in chlorella viruses

Timo Wulfmeyer, Christian Polzer, Gregor Hiepler, Kay Hamacher, Robert Shoeman, David D. Dunigan, James L. van Etten, Marco Lolicato, Anna Moroni, Gerhard Thiel, Tobias Meckel

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Chlorella viruses have icosahedral capsids with an internal membrane enclosing their large dsDNA genomes and associated proteins. Their genomes are packaged in the particles with a predicted DNA density of ca. 0.2 bp nm -3. Occasionally infection of an algal cell by an individual particle fails and the viral DNA is dynamically ejected from the capsid. This shows that the release of the DNA generates a force, which can aid in the transfer of the genome into the host in a successful infection. Imaging of ejected viral DNA indicates that it is intimately associated with proteins in a periodic fashion. The bulk of the protein particles detected by atomic force microscopy have a size of ~60 kDa and two proteins (A278L and A282L) of about this size are among 6 basic putative DNA binding proteins found in a proteomic analysis of DNA binding proteins packaged in the virion. A combination of fluorescence images of ejected DNA and a bioinformatics analysis of the DNA reveal periodic patterns in the viral DNA. The periodic distribution of GC rich regions in the genome provides potential binding sites for basic proteins. This DNA/protein aggregation could be responsible for the periodic concentration of fluorescently labeled DNA observed in ejected viral DNA. Collectively the data indicate that the large chlorella viruses have a DNA packaging strategy that differs from bacteriophages; it involves proteins and share similarities to that of chromatin structure in eukaryotes.

Original languageEnglish (US)
Article numbere30133
JournalPloS one
Volume7
Issue number2
DOIs
StatePublished - Feb 16 2012

Fingerprint

Chlorella
Viruses
Viral DNA
viruses
DNA
Genes
Genome
Proteins
Capsid
DNA-Binding Proteins
proteins
DNA-binding proteins
capsid
genome
GC Rich Sequence
DNA Packaging
Bacteriophages
Atomic Force Microscopy
Bioinformatics
Infection

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Cite this

Wulfmeyer, T., Polzer, C., Hiepler, G., Hamacher, K., Shoeman, R., Dunigan, D. D., ... Meckel, T. (2012). Structural organization of DNA in chlorella viruses. PloS one, 7(2), [e30133]. https://doi.org/10.1371/journal.pone.0030133

Structural organization of DNA in chlorella viruses. / Wulfmeyer, Timo; Polzer, Christian; Hiepler, Gregor; Hamacher, Kay; Shoeman, Robert; Dunigan, David D.; van Etten, James L.; Lolicato, Marco; Moroni, Anna; Thiel, Gerhard; Meckel, Tobias.

In: PloS one, Vol. 7, No. 2, e30133, 16.02.2012.

Research output: Contribution to journalArticle

Wulfmeyer, T, Polzer, C, Hiepler, G, Hamacher, K, Shoeman, R, Dunigan, DD, van Etten, JL, Lolicato, M, Moroni, A, Thiel, G & Meckel, T 2012, 'Structural organization of DNA in chlorella viruses', PloS one, vol. 7, no. 2, e30133. https://doi.org/10.1371/journal.pone.0030133
Wulfmeyer T, Polzer C, Hiepler G, Hamacher K, Shoeman R, Dunigan DD et al. Structural organization of DNA in chlorella viruses. PloS one. 2012 Feb 16;7(2). e30133. https://doi.org/10.1371/journal.pone.0030133
Wulfmeyer, Timo ; Polzer, Christian ; Hiepler, Gregor ; Hamacher, Kay ; Shoeman, Robert ; Dunigan, David D. ; van Etten, James L. ; Lolicato, Marco ; Moroni, Anna ; Thiel, Gerhard ; Meckel, Tobias. / Structural organization of DNA in chlorella viruses. In: PloS one. 2012 ; Vol. 7, No. 2.
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