Structural evidence for the authenticity of the human retinoblastoma gene

Yuen Kai T. Fung, A. Linn Murphree, Anne T'Ang, Jin Qian, Steven H. Hinrichs, William F. Benedict

Research output: Contribution to journalArticle

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Abstract

The retinoblastoma (Rb) gene is the prototype for a class of recessive human cancer genes in which loss of activity of both normal alleles is thought to be associated with tumorigenesis. Sixteen of 40 retinoblastomas examined with a complementary DNA probe shown to be the Rb gene had identifiable structural changes of the Rb gene including in some cases homozygous internal deletions with corresponding truncated transcripts. An osteosarcoma also had a homozygous internal deletion with a truncated transcript. In addition, possible hot spots for deletion were identified within the Rb genomic locus. Among those tumors with no identifiable structural changes locus. was eidier absence of an Rb transcript or abnormal expression of the Rb transcript. Comparison of the structural changes in the tumor cells and fibroblasts of certain patients provided support for Knudson's two-hit hypothesis for the development of retinoblastoma at the molecular level. The ability to detect germline structural deletions in fibroblasts from some patients with bilateral retinoblastoma also indicates that the isolated gene is useful for diagnostic purposes.

Original languageEnglish (US)
Pages (from-to)1657-1661
Number of pages5
JournalScience
Volume236
Issue number4809
DOIs
StatePublished - Jan 1 1987

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Retinoblastoma Genes
Retinoblastoma
Fibroblasts
Neoplasm Genes
DNA Probes
Osteosarcoma
Neoplasms
Carcinogenesis
Complementary DNA
Alleles
Genes

ASJC Scopus subject areas

  • General

Cite this

Fung, Y. K. T., Linn Murphree, A., T'Ang, A., Qian, J., Hinrichs, S. H., & Benedict, W. F. (1987). Structural evidence for the authenticity of the human retinoblastoma gene. Science, 236(4809), 1657-1661. https://doi.org/10.1126/science.2885916

Structural evidence for the authenticity of the human retinoblastoma gene. / Fung, Yuen Kai T.; Linn Murphree, A.; T'Ang, Anne; Qian, Jin; Hinrichs, Steven H.; Benedict, William F.

In: Science, Vol. 236, No. 4809, 01.01.1987, p. 1657-1661.

Research output: Contribution to journalArticle

Fung, YKT, Linn Murphree, A, T'Ang, A, Qian, J, Hinrichs, SH & Benedict, WF 1987, 'Structural evidence for the authenticity of the human retinoblastoma gene', Science, vol. 236, no. 4809, pp. 1657-1661. https://doi.org/10.1126/science.2885916
Fung YKT, Linn Murphree A, T'Ang A, Qian J, Hinrichs SH, Benedict WF. Structural evidence for the authenticity of the human retinoblastoma gene. Science. 1987 Jan 1;236(4809):1657-1661. https://doi.org/10.1126/science.2885916
Fung, Yuen Kai T. ; Linn Murphree, A. ; T'Ang, Anne ; Qian, Jin ; Hinrichs, Steven H. ; Benedict, William F. / Structural evidence for the authenticity of the human retinoblastoma gene. In: Science. 1987 ; Vol. 236, No. 4809. pp. 1657-1661.
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