Structural diversity in the type IV Pili of multidrug-resistant acinetobacter

Kurt H. Piepenbrink, Erik Lillehoj, Christian M. Harding, Jason W. Labonte, Xiaotong Zuo, Chelsea A. Rapp, Robert S. Munson, Simeon E. Goldblum, Mario F. Feldman, Jeffrey J. Gray, Eric J. Sundberg

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Acinetobacter baumannii is a Gram-negative coccobacillus found primarily in hospital settings that has recently emerged as a source of hospital-acquired infections. A. baumannii expresses a variety of virulence factors, including type IV pili, bacterial extracellular appendages often essential for attachment to host cells. Here, we report the high resolution structures of the major pilin subunit, PilA, from three Acinetobacter strains, demonstrating that A. baumannii subsets produce morphologically distinct type IV pilin glycoproteins. We examine the consequences of this heterogeneity for protein folding and assembly as well as host-cell adhesion by Acinetobacter. Comparisons of genomic and structural data with pilin proteins from other species of soil gammaproteobacteria suggest that these structural differences stem from evolutionary pressure that has resulted in three distinct classes of type IVa pilins, each found in multiple species.

Original languageEnglish (US)
Pages (from-to)22924-22935
Number of pages12
JournalJournal of Biological Chemistry
Volume291
Issue number44
DOIs
StatePublished - Oct 28 2016

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Fimbriae Proteins
Acinetobacter
Acinetobacter baumannii
Bacterial Fimbriae
Gammaproteobacteria
Protein folding
Protein Folding
Cell adhesion
Virulence Factors
Cross Infection
Cell Adhesion
Glycoproteins
Soil
Soils
Pressure
Proteins

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Piepenbrink, K. H., Lillehoj, E., Harding, C. M., Labonte, J. W., Zuo, X., Rapp, C. A., ... Sundberg, E. J. (2016). Structural diversity in the type IV Pili of multidrug-resistant acinetobacter. Journal of Biological Chemistry, 291(44), 22924-22935. https://doi.org/10.1074/jbc.M116.751099

Structural diversity in the type IV Pili of multidrug-resistant acinetobacter. / Piepenbrink, Kurt H.; Lillehoj, Erik; Harding, Christian M.; Labonte, Jason W.; Zuo, Xiaotong; Rapp, Chelsea A.; Munson, Robert S.; Goldblum, Simeon E.; Feldman, Mario F.; Gray, Jeffrey J.; Sundberg, Eric J.

In: Journal of Biological Chemistry, Vol. 291, No. 44, 28.10.2016, p. 22924-22935.

Research output: Contribution to journalArticle

Piepenbrink, KH, Lillehoj, E, Harding, CM, Labonte, JW, Zuo, X, Rapp, CA, Munson, RS, Goldblum, SE, Feldman, MF, Gray, JJ & Sundberg, EJ 2016, 'Structural diversity in the type IV Pili of multidrug-resistant acinetobacter', Journal of Biological Chemistry, vol. 291, no. 44, pp. 22924-22935. https://doi.org/10.1074/jbc.M116.751099
Piepenbrink, Kurt H. ; Lillehoj, Erik ; Harding, Christian M. ; Labonte, Jason W. ; Zuo, Xiaotong ; Rapp, Chelsea A. ; Munson, Robert S. ; Goldblum, Simeon E. ; Feldman, Mario F. ; Gray, Jeffrey J. ; Sundberg, Eric J. / Structural diversity in the type IV Pili of multidrug-resistant acinetobacter. In: Journal of Biological Chemistry. 2016 ; Vol. 291, No. 44. pp. 22924-22935.
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