Structural characterization of tachykinins (neuropeptide γ, neurokinin A, and substance P) from a reptile, Alligator mississipiensis

Yunxia Wang, Finbarr O'Harte, J. Michael Conlon

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

An extract of the whole brain of the alligator (Alligator mississipiensis) contained very high concentrations of substance P-like immunoreactivity (405 pmol/g wet tissue) and neurokinin A-like immunoreactivity (514 pmol/g), as measured with antisera raised against the mammalian peptides. The primary structure of alligator substance P was established as: Arg-Pro-Arg-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH... This sequence is the same as that of chicken substance P and shows one substitution (Arg for Lys3) as compared with mammalian substance P. The neurokinin A-like immunoreactivity was separated into two components. Neuropeptide y was the most abundant peptide and its primary structure was established as Asp-Ala-Gly-Tyr-Gly-Gln-Ile-Ser-His-Lys-Arg-His-Lys-Thr-Asp-Ser-Phe-Val-Gly-Leu-Met-NH2. This sequences shows one substitution (Tyr for His4) compared with mammalian neuropeptide y. The second component was identical to mammalian neurokinin A. A peptide with the chromatographic properties of mammalian neuropeptide K was not identified in the extract.

Original languageEnglish (US)
Pages (from-to)277-286
Number of pages10
JournalGeneral and Comparative Endocrinology
Volume88
Issue number2
DOIs
StatePublished - Nov 1992

Fingerprint

Neurokinin A
Alligators and Crocodiles
Tachykinins
alligators
substance P
Reptiles
neuropeptides
Substance P
Neuropeptides
reptiles
peptides
Peptides
extracts
antiserum
Immune Sera
Chickens
chickens
brain
Brain

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Endocrinology

Cite this

Structural characterization of tachykinins (neuropeptide γ, neurokinin A, and substance P) from a reptile, Alligator mississipiensis. / Wang, Yunxia; O'Harte, Finbarr; Conlon, J. Michael.

In: General and Comparative Endocrinology, Vol. 88, No. 2, 11.1992, p. 277-286.

Research output: Contribution to journalArticle

@article{766f448dd46e4547bfd14cd871fd54ff,
title = "Structural characterization of tachykinins (neuropeptide γ, neurokinin A, and substance P) from a reptile, Alligator mississipiensis",
abstract = "An extract of the whole brain of the alligator (Alligator mississipiensis) contained very high concentrations of substance P-like immunoreactivity (405 pmol/g wet tissue) and neurokinin A-like immunoreactivity (514 pmol/g), as measured with antisera raised against the mammalian peptides. The primary structure of alligator substance P was established as: Arg-Pro-Arg-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH... This sequence is the same as that of chicken substance P and shows one substitution (Arg for Lys3) as compared with mammalian substance P. The neurokinin A-like immunoreactivity was separated into two components. Neuropeptide y was the most abundant peptide and its primary structure was established as Asp-Ala-Gly-Tyr-Gly-Gln-Ile-Ser-His-Lys-Arg-His-Lys-Thr-Asp-Ser-Phe-Val-Gly-Leu-Met-NH2. This sequences shows one substitution (Tyr for His4) compared with mammalian neuropeptide y. The second component was identical to mammalian neurokinin A. A peptide with the chromatographic properties of mammalian neuropeptide K was not identified in the extract.",
author = "Yunxia Wang and Finbarr O'Harte and Conlon, {J. Michael}",
year = "1992",
month = "11",
doi = "10.1016/0016-6480(92)90260-Q",
language = "English (US)",
volume = "88",
pages = "277--286",
journal = "General and Comparative Endocrinology",
issn = "0016-6480",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Structural characterization of tachykinins (neuropeptide γ, neurokinin A, and substance P) from a reptile, Alligator mississipiensis

AU - Wang, Yunxia

AU - O'Harte, Finbarr

AU - Conlon, J. Michael

PY - 1992/11

Y1 - 1992/11

N2 - An extract of the whole brain of the alligator (Alligator mississipiensis) contained very high concentrations of substance P-like immunoreactivity (405 pmol/g wet tissue) and neurokinin A-like immunoreactivity (514 pmol/g), as measured with antisera raised against the mammalian peptides. The primary structure of alligator substance P was established as: Arg-Pro-Arg-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH... This sequence is the same as that of chicken substance P and shows one substitution (Arg for Lys3) as compared with mammalian substance P. The neurokinin A-like immunoreactivity was separated into two components. Neuropeptide y was the most abundant peptide and its primary structure was established as Asp-Ala-Gly-Tyr-Gly-Gln-Ile-Ser-His-Lys-Arg-His-Lys-Thr-Asp-Ser-Phe-Val-Gly-Leu-Met-NH2. This sequences shows one substitution (Tyr for His4) compared with mammalian neuropeptide y. The second component was identical to mammalian neurokinin A. A peptide with the chromatographic properties of mammalian neuropeptide K was not identified in the extract.

AB - An extract of the whole brain of the alligator (Alligator mississipiensis) contained very high concentrations of substance P-like immunoreactivity (405 pmol/g wet tissue) and neurokinin A-like immunoreactivity (514 pmol/g), as measured with antisera raised against the mammalian peptides. The primary structure of alligator substance P was established as: Arg-Pro-Arg-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH... This sequence is the same as that of chicken substance P and shows one substitution (Arg for Lys3) as compared with mammalian substance P. The neurokinin A-like immunoreactivity was separated into two components. Neuropeptide y was the most abundant peptide and its primary structure was established as Asp-Ala-Gly-Tyr-Gly-Gln-Ile-Ser-His-Lys-Arg-His-Lys-Thr-Asp-Ser-Phe-Val-Gly-Leu-Met-NH2. This sequences shows one substitution (Tyr for His4) compared with mammalian neuropeptide y. The second component was identical to mammalian neurokinin A. A peptide with the chromatographic properties of mammalian neuropeptide K was not identified in the extract.

UR - http://www.scopus.com/inward/record.url?scp=0026738012&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026738012&partnerID=8YFLogxK

U2 - 10.1016/0016-6480(92)90260-Q

DO - 10.1016/0016-6480(92)90260-Q

M3 - Article

C2 - 1282482

AN - SCOPUS:0026738012

VL - 88

SP - 277

EP - 286

JO - General and Comparative Endocrinology

JF - General and Comparative Endocrinology

SN - 0016-6480

IS - 2

ER -