Structural basis of Ets1 cooperative binding to widely separated sites on promoter DNA

Nigar D. Babayeva, Oxana I. Baranovskaya, Tahir H Tahirov

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Ets1 is a member of the Ets family of transcription factors. Ets1 is expressed in autoinhibited form and its DNA binding depends on partner proteins bound to adjacent sequences or the relative positioning of a second Ets-binding site (EBS). The autoinhibition of Ets1 is mediated by structural coupling of regions flanking the DNA-binding domain. The NMR structure of Ets1 revealed that the inhibitory regions comprised of helices HI1 and HI2 and H4 are packed together on the Ets domain to form an inhibitory module. The crystal structure of Ets1 unexpectedly revealed a homodimer in which homodimerisation occurs via swapping of HI1 helices. Modeling of DNA binding indicates that the Ets1 dimer can bind to two antiparallel pieces of DNA. To verify this, we crystallized and solved the structure of the complex comprised of Ets1 dimer and two pieces of DNA. DNA binding by Ets1 dimer resulted in formation of additional intermolecular protein•DNA interactions, implying that the complex formation is cooperative.

Original languageEnglish (US)
Article numbere33698
JournalPloS one
Volume7
Issue number3
DOIs
StatePublished - Mar 14 2012

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cooperatives
promoter regions
DNA
Dimers
Proto-Oncogene Proteins c-ets
DNA-binding domains
crystal structure
binding sites
transcription factors
Transcription Factors
Crystal structure
Binding Sites
Nuclear magnetic resonance
proteins
Proteins

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Cite this

Structural basis of Ets1 cooperative binding to widely separated sites on promoter DNA. / Babayeva, Nigar D.; Baranovskaya, Oxana I.; Tahirov, Tahir H.

In: PloS one, Vol. 7, No. 3, e33698, 14.03.2012.

Research output: Contribution to journalArticle

Babayeva, Nigar D. ; Baranovskaya, Oxana I. ; Tahirov, Tahir H. / Structural basis of Ets1 cooperative binding to widely separated sites on promoter DNA. In: PloS one. 2012 ; Vol. 7, No. 3.
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