Structural basis for transcription elongation by bacterial RNA polymerase

Dmitry G. Vassylyev, Marina N. Vassylyeva, Anna Perederina, Tahir H Tahirov, Irina Artsimovitch

Research output: Contribution to journalArticle

300 Citations (Scopus)

Abstract

The RNA polymerase elongation complex (EC) is both highly stable and processive, rapidly extending RNA chains for thousands of nucleotides. Understanding the mechanisms of elongation and its regulation requires detailed information about the structural organization of the EC. Here we report the 2.5-Å resolution structure of the Thermus thermophilus EC; the structure reveals the post-translocated intermediate with the DNA template in the active site available for pairing with the substrate. DNA strand separation occurs one position downstream of the active site, implying that only one substrate at a time can specifically bind to the EC. The upstream edge of the RNA/DNA hybrid stacks on the β′-subunit 'lid' loop, whereas the first displaced RNA base is trapped within a protein pocket, suggesting a mechanism for RNA displacement. The RNA is threaded through the RNA exit channel, where it adopts a conformation mimicking that of a single strand within a double helix, providing insight into a mechanism for hairpin-dependent pausing and termination.

Original languageEnglish (US)
Pages (from-to)157-162
Number of pages6
JournalNature
Volume448
Issue number7150
DOIs
StatePublished - Jul 12 2007

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Bacterial RNA
DNA-Directed RNA Polymerases
RNA
DNA
Catalytic Domain
Thermus thermophilus
Nucleotides

ASJC Scopus subject areas

  • General

Cite this

Vassylyev, D. G., Vassylyeva, M. N., Perederina, A., Tahirov, T. H., & Artsimovitch, I. (2007). Structural basis for transcription elongation by bacterial RNA polymerase. Nature, 448(7150), 157-162. https://doi.org/10.1038/nature05932

Structural basis for transcription elongation by bacterial RNA polymerase. / Vassylyev, Dmitry G.; Vassylyeva, Marina N.; Perederina, Anna; Tahirov, Tahir H; Artsimovitch, Irina.

In: Nature, Vol. 448, No. 7150, 12.07.2007, p. 157-162.

Research output: Contribution to journalArticle

Vassylyev, DG, Vassylyeva, MN, Perederina, A, Tahirov, TH & Artsimovitch, I 2007, 'Structural basis for transcription elongation by bacterial RNA polymerase', Nature, vol. 448, no. 7150, pp. 157-162. https://doi.org/10.1038/nature05932
Vassylyev DG, Vassylyeva MN, Perederina A, Tahirov TH, Artsimovitch I. Structural basis for transcription elongation by bacterial RNA polymerase. Nature. 2007 Jul 12;448(7150):157-162. https://doi.org/10.1038/nature05932
Vassylyev, Dmitry G. ; Vassylyeva, Marina N. ; Perederina, Anna ; Tahirov, Tahir H ; Artsimovitch, Irina. / Structural basis for transcription elongation by bacterial RNA polymerase. In: Nature. 2007 ; Vol. 448, No. 7150. pp. 157-162.
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