Structural and functional conservation at the boundaries of the chicken β-globin domain

Noriko Saitoh, Adam C. Bell, Félix Recillas-Targa, Adam G. West, Melanie A Simpson, Michael Pikaart, Gary Felsenfeld

Research output: Contribution to journalArticle

129 Citations (Scopus)

Abstract

We show that the 3' boundary of the chicken β-globin locus bears striking structural similarities to the 5' boundary. In erythroid cells a clear transition in DNase I sensitivity of chromatin at the 3' end of the locus is observed, the location of this transition is marked by a constitutive DNase I hypersensitive site (HS), and DNA spanning this site has the enhancer-blocking capacity of an insulator. This HS contains a binding site for the transcription factor CTCF. As in the case of the 5' insulator, the CTCF site is both necessary and sufficient for the enhancer-blocking activity of the 3' boundary. The position of this insulator is consistent with our proposal that it may function to maintain the distinct regulatory programs of the globin genes and their closely appended 3' neighbor, an odorant receptor gene. We conclude that both boundaries of the chicken β-globin domain are capable of playing functionally similar roles and that the same protein is a necessary component of the molecular mechanism through which these boundaries are defined.

Original languageEnglish (US)
Pages (from-to)2315-2322
Number of pages8
JournalEMBO Journal
Volume19
Issue number10
DOIs
StatePublished - May 15 2000

Fingerprint

Globins
Chickens
Conservation
Deoxyribonuclease I
Genes
Odorant Receptors
Erythroid Cells
Chromatin
Transcription Factors
Binding Sites
DNA
Proteins

Keywords

  • CTCF
  • Chromatin domains
  • Globin genes
  • Insulators
  • Odorant receptors

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Saitoh, N., Bell, A. C., Recillas-Targa, F., West, A. G., Simpson, M. A., Pikaart, M., & Felsenfeld, G. (2000). Structural and functional conservation at the boundaries of the chicken β-globin domain. EMBO Journal, 19(10), 2315-2322. https://doi.org/10.1093/emboj/19.10.2315

Structural and functional conservation at the boundaries of the chicken β-globin domain. / Saitoh, Noriko; Bell, Adam C.; Recillas-Targa, Félix; West, Adam G.; Simpson, Melanie A; Pikaart, Michael; Felsenfeld, Gary.

In: EMBO Journal, Vol. 19, No. 10, 15.05.2000, p. 2315-2322.

Research output: Contribution to journalArticle

Saitoh, N, Bell, AC, Recillas-Targa, F, West, AG, Simpson, MA, Pikaart, M & Felsenfeld, G 2000, 'Structural and functional conservation at the boundaries of the chicken β-globin domain', EMBO Journal, vol. 19, no. 10, pp. 2315-2322. https://doi.org/10.1093/emboj/19.10.2315
Saitoh N, Bell AC, Recillas-Targa F, West AG, Simpson MA, Pikaart M et al. Structural and functional conservation at the boundaries of the chicken β-globin domain. EMBO Journal. 2000 May 15;19(10):2315-2322. https://doi.org/10.1093/emboj/19.10.2315
Saitoh, Noriko ; Bell, Adam C. ; Recillas-Targa, Félix ; West, Adam G. ; Simpson, Melanie A ; Pikaart, Michael ; Felsenfeld, Gary. / Structural and functional conservation at the boundaries of the chicken β-globin domain. In: EMBO Journal. 2000 ; Vol. 19, No. 10. pp. 2315-2322.
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