Structural and evolutionary analyses show unique stabilization strategies in the type IV Pili of clostridium difficile

Kurt H Piepenbrink, Grace A. Maldarelli, Claudia F. Martinez De La Peña, Tanis C. Dingle, George L. Mulvey, Amanda Lee, Erik Von Rosenvinge, Glen D. Armstrong, Michael S. Donnenberg, Eric J. Sundberg

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Type IV pili are produced by many pathogenic Gram-negative bacteria and are important for processes as diverse as twitching motility, biofilm formation, cellular adhesion, and horizontal gene transfer. However, many Gram-positive species, including Clostridium difficile, also produce type IV pili. Here, we identify the major subunit of the type IV pili of C. difficile, PilA1, and describe multiple 3D structures of PilA1, demonstrating the diversity found in three strains of C. difficile. We also model the incorporation of both PilA1 and a minor pilin, PilJ, into the pilus fiber. Although PilA1 contains no cysteine residues, and therefore cannot form the disulfide bonds found in all Gram-negative type IV pilins, it adopts unique strategies to achieve a typical pilin fold. The structures of PilA1 and PilJ exhibit similarities with the type IVb pilins from Gram-negative bacteria that suggest that the type IV pili of C. difficile are involved in microcolony formation.

Original languageEnglish (US)
Pages (from-to)385-396
Number of pages12
JournalStructure
Volume23
Issue number2
DOIs
StatePublished - Feb 3 2015

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Fimbriae Proteins
Clostridium difficile
Gram-Negative Bacteria
Horizontal Gene Transfer
Biofilms
Disulfides
Cysteine

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

Cite this

Piepenbrink, K. H., Maldarelli, G. A., Martinez De La Peña, C. F., Dingle, T. C., Mulvey, G. L., Lee, A., ... Sundberg, E. J. (2015). Structural and evolutionary analyses show unique stabilization strategies in the type IV Pili of clostridium difficile. Structure, 23(2), 385-396. https://doi.org/10.1016/j.str.2014.11.018

Structural and evolutionary analyses show unique stabilization strategies in the type IV Pili of clostridium difficile. / Piepenbrink, Kurt H; Maldarelli, Grace A.; Martinez De La Peña, Claudia F.; Dingle, Tanis C.; Mulvey, George L.; Lee, Amanda; Von Rosenvinge, Erik; Armstrong, Glen D.; Donnenberg, Michael S.; Sundberg, Eric J.

In: Structure, Vol. 23, No. 2, 03.02.2015, p. 385-396.

Research output: Contribution to journalArticle

Piepenbrink, KH, Maldarelli, GA, Martinez De La Peña, CF, Dingle, TC, Mulvey, GL, Lee, A, Von Rosenvinge, E, Armstrong, GD, Donnenberg, MS & Sundberg, EJ 2015, 'Structural and evolutionary analyses show unique stabilization strategies in the type IV Pili of clostridium difficile', Structure, vol. 23, no. 2, pp. 385-396. https://doi.org/10.1016/j.str.2014.11.018
Piepenbrink, Kurt H ; Maldarelli, Grace A. ; Martinez De La Peña, Claudia F. ; Dingle, Tanis C. ; Mulvey, George L. ; Lee, Amanda ; Von Rosenvinge, Erik ; Armstrong, Glen D. ; Donnenberg, Michael S. ; Sundberg, Eric J. / Structural and evolutionary analyses show unique stabilization strategies in the type IV Pili of clostridium difficile. In: Structure. 2015 ; Vol. 23, No. 2. pp. 385-396.
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