Structural analyses of Phycodnaviridae and Iridoviridae

Alan A. Simpson, Narayanasamy Nandhagopal, James L Van Etten, Michael G. Rossmann

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

The Phycodnaviridae, Iridoviridae and related viruses, with diameters of 1500-2000 Å, are formed from large trigonal arrays of hexagonally close-packed capsomers forming the faces of icosahedra [Yan et al. (2000), Nature Struct. Biol. 7, 101-103; Nandhagopal et al. (2002), Proc. Natl Acad. Sci. USA, 99, 14758-14763]. Caspar and Klug predicted that such structures could be assembled from hexameric capsomers [Caspar & Klug (1962), Cold Spring Harbor. Symp. Quant. Biol. 27, 1-24], as was subsequently found in numerous icosahedral viruses. During the course of evolution, some viruses, including the virus families mentioned above, replaced hexameric capsomers with pseudo-hexameric trimers by gene duplication. In large dsDNA icosahedral viruses, the capsomers are organized into 'pentasymmetrons' and 'trisymmetrons'. The interactions between the trimeric capsomers can be divided into three groups, one between similarly oriented trimers and two between oppositely oriented trimers (trimers related by an approximately sixfold rotation). The interactions within a trisymmetron belong to the first class, whereas those between trisymmetrons and within the pentasymmetron are of the other two types. Knowledge of these distances permits a more accurate fitting of the atomic structure of the capsomer into the cryo-electron microscopy (cryoEM) reconstruction of the whole virus. The adoption of pseudo-hexagonal capsomers places these viruses into a subset of the Caspar and Klug surface lattices.

Original languageEnglish (US)
Pages (from-to)2053-2059
Number of pages7
JournalActa Crystallographica - Section D Biological Crystallography
Volume59
Issue number12
DOIs
StatePublished - Dec 1 2003

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Phycodnaviridae
Iridoviridae
viruses
Viruses
trimers
Cryoelectron Microscopy
Gene Duplication
harbors
Ports and harbors
atomic structure
genes
Electron microscopy
set theory
electron microscopy
Genes
interactions

ASJC Scopus subject areas

  • Structural Biology
  • Medicine(all)

Cite this

Structural analyses of Phycodnaviridae and Iridoviridae. / Simpson, Alan A.; Nandhagopal, Narayanasamy; Van Etten, James L; Rossmann, Michael G.

In: Acta Crystallographica - Section D Biological Crystallography, Vol. 59, No. 12, 01.12.2003, p. 2053-2059.

Research output: Contribution to journalArticle

Simpson, Alan A. ; Nandhagopal, Narayanasamy ; Van Etten, James L ; Rossmann, Michael G. / Structural analyses of Phycodnaviridae and Iridoviridae. In: Acta Crystallographica - Section D Biological Crystallography. 2003 ; Vol. 59, No. 12. pp. 2053-2059.
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