Stromal Cell-Derived Factor 1-Mediated CXCR4 Signaling in Rat and Human Cortical Neural Progenitor Cells

Hui Peng, Yunlong Huang, Jeremy Rose, David Erichsen, Shelley Herek, Nobutaka Fujii, Hirokazu Tamamura, Jialin C Zheng

Research output: Contribution to journalArticle

142 Citations (Scopus)

Abstract

Stromal cell-derived factor 1 (SDF-1) and the chemokine receptor CXCR4 are highly expressed in the nervous system. Knockout studies have suggested that both SDF-1 and CXCR4 play essential roles in cerebellar, hippocampal, and neocortical neural cell migration during embryogenesis. To extend these observations, CXCR4 signaling events in rat and human neural progenitor cells (NPCs) were examined. Our results show that CXCR4 is expressed in abundance on rat and human NPCs. Moreover, SDF-1α induced increased NPCs levels of inositol 1,4,5-triphosphate, extracellular signal-regulated kinases 1/2, Akt, c-Jun N-terminal kinase, and intracellular calcium whereas it diminished cyclic adenosine monophosphate. Finally, SDF-1α can induce human NPC chemotaxis in vitro, suggesting that CXCR4 plays a functional role in NPC migration. Both T140, a CXCR4 antagonist, and pertussis toxin (PTX), an inactivator of G protein-coupled receptors, abrogated these events. Ultimately, this study suggested that SDF-1α can influence NPC function through CXCR4 and that CXCR4 is functional on NPC.

Original languageEnglish (US)
Pages (from-to)35-50
Number of pages16
JournalJournal of Neuroscience Research
Volume76
Issue number1
DOIs
StatePublished - Apr 1 2004

Fingerprint

Chemokine CXCL12
Stem Cells
Cell Movement
Inositol 1,4,5-Trisphosphate
Mitogen-Activated Protein Kinase 3
JNK Mitogen-Activated Protein Kinases
Chemokine Receptors
Mitogen-Activated Protein Kinase 1
Pertussis Toxin
Chemotaxis
G-Protein-Coupled Receptors
Cyclic AMP
Nervous System
Embryonic Development
Calcium

Keywords

  • CXCR4
  • Intracellular signaling
  • Neural progenitor cell
  • Neurogenesis
  • SDF-1α

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

Cite this

Stromal Cell-Derived Factor 1-Mediated CXCR4 Signaling in Rat and Human Cortical Neural Progenitor Cells. / Peng, Hui; Huang, Yunlong; Rose, Jeremy; Erichsen, David; Herek, Shelley; Fujii, Nobutaka; Tamamura, Hirokazu; Zheng, Jialin C.

In: Journal of Neuroscience Research, Vol. 76, No. 1, 01.04.2004, p. 35-50.

Research output: Contribution to journalArticle

Peng, Hui ; Huang, Yunlong ; Rose, Jeremy ; Erichsen, David ; Herek, Shelley ; Fujii, Nobutaka ; Tamamura, Hirokazu ; Zheng, Jialin C. / Stromal Cell-Derived Factor 1-Mediated CXCR4 Signaling in Rat and Human Cortical Neural Progenitor Cells. In: Journal of Neuroscience Research. 2004 ; Vol. 76, No. 1. pp. 35-50.
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