Strial marginal cells play a role in basement membrane homeostasis

In vitro and in vivo evidence

Michael Anne Gratton, Daniel T. Meehan, Brendan J. Smyth, Dominic E Cosgrove

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The interaction of extracellular matrix and receptors plays a role in tissue homeostasis. The thickened strial capillary basement membrane (SCBM) reported in animal models of presbycusis and Alport's syndrome might be secondary to elevated synthesis and/or decreased turnover of specific basement membrane (BM) components. In this study, expression of specific BM proteins, integrin receptors and mediators of matrix turnover in the murine lateral wall were determined using cDNA probes and antibodies. The presence of collagen α1 and α2(IV) and laminin-8 in the SCBM was verified. The integrin subunits α3, αv and β1, cell surface receptors for the BM proteins, localized primarily to the SCBM and/or the strial marginal cells as did TIMP-3, a tissue inhibitor of matrix metalloproteinase. The epithelial cell line SV-k1, derived from the lateral wall of the 'immortomouse', showed expression of the same BM proteins as well as demonstrating the presence of markers specific to strial marginal cells, namely Na,K-ATPase α1 and β2 subunits. Thus, the cultured cells are identified as deriving from marginal cells of the stria vascularis. Moreover, these data suggest that a culture system using this marginal cell line will be useful to delineate mechanisms underlying the pathologic accumulation of extracellular matrix in the SCBM.

Original languageEnglish (US)
Pages (from-to)27-36
Number of pages10
JournalHearing Research
Volume163
Issue number1-2
DOIs
StatePublished - Feb 5 2002

Fingerprint

Basement Membrane
Homeostasis
Membrane Proteins
Integrins
Presbycusis
Tissue Inhibitor of Metalloproteinase-3
Hereditary Nephritis
Stria Vascularis
Tissue Inhibitor of Metalloproteinases
Cell Line
In Vitro Techniques
Cell Surface Receptors
Extracellular Matrix
Cultured Cells
Collagen
Animal Models
Complementary DNA
Epithelial Cells
Antibodies

Keywords

  • Basement membrane
  • Cell culture
  • Cochlea
  • Extracellular matrix
  • Pathogenesis
  • Stria vascularis

ASJC Scopus subject areas

  • Sensory Systems

Cite this

Strial marginal cells play a role in basement membrane homeostasis : In vitro and in vivo evidence. / Gratton, Michael Anne; Meehan, Daniel T.; Smyth, Brendan J.; Cosgrove, Dominic E.

In: Hearing Research, Vol. 163, No. 1-2, 05.02.2002, p. 27-36.

Research output: Contribution to journalArticle

Gratton, Michael Anne ; Meehan, Daniel T. ; Smyth, Brendan J. ; Cosgrove, Dominic E. / Strial marginal cells play a role in basement membrane homeostasis : In vitro and in vivo evidence. In: Hearing Research. 2002 ; Vol. 163, No. 1-2. pp. 27-36.
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