Stress-activated protein kinases are involved in coxsackievirus B3 viral progeny release

Xiaoning Si, Honglin Luo, Andrew Morgan, Jingchun Zhang, Jerry Wong, Ji Yuan, Mitra Esfandiarei, Guang Gao, Caroline Cheung, Bruce M. McManus

Research output: Contribution to journalArticle

83 Citations (Scopus)

Abstract

Stress-activated protein kinases (SAPKs), consisting of c-Jun N-terminal kinase (JNK) and p38 mitogen-activated protein kinase (p38 MAPK), are activated upon various environmental stimuli, including viral infections. Cellular survival and death signaling events following coxsackievirus B3 (CVB3) infection have been studied in relationship to viral replication, but the role of SAPKs has not been scrutinized. IE this study, we found that the phosphorylation of JNK1/2 and p38 MAPK was increased during active replication of CVB3 and that their phosphorylation was independent of CVB3-induced caspase activation or production of reactive oxygen species. The roles of these kinases in CVB3 infection were further evaluated using specific inhibitors: SP600125 for JNK1/2 and SB203580 for p38 MAPIC JNK1/2 inhibitors reduced CVB3-induced phosphorylation of activating transcription factor 2, and the p38 MAPK inhibitor reduced CVB3-induced phosphorylation of heat shock protein 27. Although inhibition of these kinases by specific inhibitors did not affect CVB3 viral protein synthesis, inhibition of p38 MAPK but not of JNK1/2 resulted in significant reduction of viral progeny release, suppression of CVB3-induced cell death, and blockage of CVB3-induced caspase-3 activation in infected cells. We conclude that SAPK pathways play critical roles in the life cycle of CVB3, particularly in viral progeny release.

Original languageEnglish (US)
Pages (from-to)13875-13881
Number of pages7
JournalJournal of virology
Volume79
Issue number22
DOIs
StatePublished - Nov 1 2005

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Enterovirus
Heat-Shock Proteins
mitogen-activated protein kinase
Protein Kinases
p38 Mitogen-Activated Protein Kinases
Phosphorylation
Coxsackievirus Infections
phosphorylation
Phosphotransferases
Activating Transcription Factor 2
HSP27 Heat-Shock Proteins
phosphotransferases (kinases)
Critical Pathways
JNK Mitogen-Activated Protein Kinases
infection
Viral Proteins
Virus Diseases
Protein Kinase Inhibitors
Caspases
Life Cycle Stages

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

Stress-activated protein kinases are involved in coxsackievirus B3 viral progeny release. / Si, Xiaoning; Luo, Honglin; Morgan, Andrew; Zhang, Jingchun; Wong, Jerry; Yuan, Ji; Esfandiarei, Mitra; Gao, Guang; Cheung, Caroline; McManus, Bruce M.

In: Journal of virology, Vol. 79, No. 22, 01.11.2005, p. 13875-13881.

Research output: Contribution to journalArticle

Si, X, Luo, H, Morgan, A, Zhang, J, Wong, J, Yuan, J, Esfandiarei, M, Gao, G, Cheung, C & McManus, BM 2005, 'Stress-activated protein kinases are involved in coxsackievirus B3 viral progeny release', Journal of virology, vol. 79, no. 22, pp. 13875-13881. https://doi.org/10.1128/JVI.79.22.13875-13881.2005
Si, Xiaoning ; Luo, Honglin ; Morgan, Andrew ; Zhang, Jingchun ; Wong, Jerry ; Yuan, Ji ; Esfandiarei, Mitra ; Gao, Guang ; Cheung, Caroline ; McManus, Bruce M. / Stress-activated protein kinases are involved in coxsackievirus B3 viral progeny release. In: Journal of virology. 2005 ; Vol. 79, No. 22. pp. 13875-13881.
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