Stimulation through the T cell receptor leads to interactions between SHB and several signaling proteins

Michael Welsh, Zhou Songyang, J. Daniel Frantz, Thomas Trüb, Kris A. Reedquist, Torbjörn Karlsson, Masaya Miyazaki, Lewis C. Cantley, Hamid Band, Steven E. Shoelson

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Shb is a recently described Src homology 2 (SH2) domain-containing adaptor protein. Here we show that Shb is expressed in lymphoid tissues, and is recruited into signaling complexes upon activation of Jurkat T cells. Grb2 binds proline-rich motifs in Shb via its SH3 domains. As a result, a number of proteins detected in anti-Shb and anti-Grb2 immunoprecipitates are shared, including phosphoproteins of 22, 36/38, 55/57 and 70 kDa. Shb-association with p22, which represents the T cell receptor associated ζ chain, occurs through the Shb SH2 domain. The central region of Shb binds p36/38. Since this interaction was inhibited by phosphotyrosine, this region of Shb is likely to contain a non-SH2 PTB (phosphotyrosine binding) domain. The Shb PTB domain was found to preferentially bind the sequence Asp-Asp-X-pTyr when incubated with a phosphopeptide library. A peptide corresponding to a phosphorylation site in 34 kDa Lnk inhibited association between Shb and p36/38. Overexpression of Shb in Jurkat cells led to increased basal phosphorylation of Shb-associated p36/38 and p70 proteins. Inactivation of the Shb SH2 domain by an R522K mutation resulted in a reduced stimulation of tyrosine phosphorylation of several proteins in response to CD3 crosslinking when expressed in Jurkat cells. Together, our results show three distinct domains of Shb all participate in the formulation of multimeric signaling complexes in activated T cells. These results indicate that the Shb protein functions in T cell receptor signaling.

Original languageEnglish (US)
Pages (from-to)891-901
Number of pages11
JournalOncogene
Volume16
Issue number7
DOIs
StatePublished - Feb 19 1998
Externally publishedYes

Fingerprint

T-Cell Antigen Receptor
src Homology Domains
Phosphotyrosine
Jurkat Cells
Phosphorylation
Proteins
aspartyl-aspartic acid
T-Lymphocytes
Phosphopeptides
Phosphoproteins
Lymphoid Tissue
Proline
Tyrosine
Peptides
Mutation

Keywords

  • Jurkat T cells
  • PTB domain
  • SH2 domain
  • Shb
  • T cell receptor
  • p36/38

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

Cite this

Welsh, M., Songyang, Z., Frantz, J. D., Trüb, T., Reedquist, K. A., Karlsson, T., ... Shoelson, S. E. (1998). Stimulation through the T cell receptor leads to interactions between SHB and several signaling proteins. Oncogene, 16(7), 891-901. https://doi.org/10.1038/sj.onc.1201607

Stimulation through the T cell receptor leads to interactions between SHB and several signaling proteins. / Welsh, Michael; Songyang, Zhou; Frantz, J. Daniel; Trüb, Thomas; Reedquist, Kris A.; Karlsson, Torbjörn; Miyazaki, Masaya; Cantley, Lewis C.; Band, Hamid; Shoelson, Steven E.

In: Oncogene, Vol. 16, No. 7, 19.02.1998, p. 891-901.

Research output: Contribution to journalArticle

Welsh, M, Songyang, Z, Frantz, JD, Trüb, T, Reedquist, KA, Karlsson, T, Miyazaki, M, Cantley, LC, Band, H & Shoelson, SE 1998, 'Stimulation through the T cell receptor leads to interactions between SHB and several signaling proteins', Oncogene, vol. 16, no. 7, pp. 891-901. https://doi.org/10.1038/sj.onc.1201607
Welsh M, Songyang Z, Frantz JD, Trüb T, Reedquist KA, Karlsson T et al. Stimulation through the T cell receptor leads to interactions between SHB and several signaling proteins. Oncogene. 1998 Feb 19;16(7):891-901. https://doi.org/10.1038/sj.onc.1201607
Welsh, Michael ; Songyang, Zhou ; Frantz, J. Daniel ; Trüb, Thomas ; Reedquist, Kris A. ; Karlsson, Torbjörn ; Miyazaki, Masaya ; Cantley, Lewis C. ; Band, Hamid ; Shoelson, Steven E. / Stimulation through the T cell receptor leads to interactions between SHB and several signaling proteins. In: Oncogene. 1998 ; Vol. 16, No. 7. pp. 891-901.
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