Stimulation of the cyclooxygenase activity of prostaglandin H synthase by salicylate-derived quinhydrones

T. J. Holmes, V. John, J. Vennerstrom, Richard J. Kulmacz, William E.M. Lands

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Two novel salicylate-derived quinhydrones were evaluated for their effect on the kinetics of cyclooxygenase activity of sheep seminal vesicle prostaglandin H synthase. These quinhydrones, which form semiquinone radicals in solution, were designed to resemble oxidized intermediates of salicylic acid metabolism. Although initially investigated for their potential role in irreversible inactivation of cyclooxygenase, these derivatives were found to give three-fold stimulation of this activity. In the absence of arachidonic acid, preincubation of the enzyme with these quinhydrones did not lead to inactivation of the cyclooxygenase activity. These compounds thus resemble the phenolic antioxidants in their effects on the cyclooxygenase activity of the synthase.

Original languageEnglish (US)
Pages (from-to)711-716
Number of pages6
JournalProstaglandins
Volume28
Issue number5
DOIs
StatePublished - Nov 1984

Fingerprint

Salicylates
Prostaglandin-Endoperoxide Synthases
Salicylic Acid
Seminal Vesicles
Arachidonic Acid
Metabolism
Sheep
Antioxidants
quinhydrone
Derivatives
Kinetics
Enzymes

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology

Cite this

Stimulation of the cyclooxygenase activity of prostaglandin H synthase by salicylate-derived quinhydrones. / Holmes, T. J.; John, V.; Vennerstrom, J.; Kulmacz, Richard J.; Lands, William E.M.

In: Prostaglandins, Vol. 28, No. 5, 11.1984, p. 711-716.

Research output: Contribution to journalArticle

Holmes, T. J. ; John, V. ; Vennerstrom, J. ; Kulmacz, Richard J. ; Lands, William E.M. / Stimulation of the cyclooxygenase activity of prostaglandin H synthase by salicylate-derived quinhydrones. In: Prostaglandins. 1984 ; Vol. 28, No. 5. pp. 711-716.
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