Stimulation of phospholipid labeling and steroidogenesis by luteinizing hormone in isolated bovine luteal cells

John S. Davis, Robert V. Farese, John M. Marsh

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Abstract

The present study was conducted to examine the effect of LH on phospholipid metabolism in corpora luteal tissue. Collagenase-dispersed cells obtained from bovine corpora lutea of early pregnancy were incubated with 32PO4 in the presence or absence of LH and examined for their ability to incorporate this label into phospholipids. LH (1 μg/ml) significantly increased 32P incorporation into total lipid extracts, with a time course similar to that of progesterone synthesis. This stimulation of 32P incorporation was dependent on the concentration of LH, and this dose-response relationship correlated well with the dose response of LH-induced progesterone production. Bovine serum albumin and ACTH had no apparent effect on 32P incorporation into phospholipids or progesterone production. Separation of luteal cell phospholipid extracts by thin layer chromatography revealed that LH stimulated the incorporation 32P4, mainly into pho phatidic acid and phosphatidylinositol, with small increases occurring in the polyphosphoinositide fraction. The LHinduced labeling of these individual phospholipids also appeared to be temporally and dose-related to the LH-induced increases in progesterone synthesis. LH had no effect on the labeling of phosphatidylcholine, phosphatidylserine, sphingomyelin, or cardiolipin. These results indicate t at LH has selective effects on phospholipid metabolism in bovine luteal cells which may be a part of the mechanism of action of LH on steroidogenesis.

Original languageEnglish (US)
Pages (from-to)469-475
Number of pages7
JournalEndocrinology
Volume109
Issue number2
DOIs
Publication statusPublished - Aug 1981

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ASJC Scopus subject areas

  • Endocrinology

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