Stimulation of ovarian tumor cell proliferation with monocyte products including interleukin-1, interleukin-6, and tumor necrosis factor-α

Shu Wu, Kerry Rodabaugh, Otoniel Martinez-Maza, Joanna M. Watson, David S. Silberstein, Cinda M. Boyer, William P. Peters, J. Brice Weinberg, Jonathan S. Berek, Robert C. Bast

Research output: Contribution to journalArticle

91 Citations (Scopus)

Abstract

Objective: We investigated whether monocyte-derived factors could stimulate the growth of ovarian cancer cells. Study Design: Human peripheral blood monocytes or human monocyte-like cell lines THP-1 and U-937 were cultured with or without macrophage colony-stimulating factor, lipopolysaccharide, or phorbol myristate acetate. Culture supernatants or recombinant cytokines were assayed for growth stimulation of ovarian cancer cell lines by tritium-thymidine incorporation and direct cell counts followed by statistical analysis with Student t test. Results: Conditioned medium from peripheral blood monocytes or from THP-1 or U-937 cells stimulated ovarian cancer cell growth. Interleukin-1α, tumor necrosis factor-α, and interleukin-6 also stimulated ovarian cancer cell growth, whereas macrophage, granulocyte, and granulocyte-macrophage colony-stimulating factor did not. Concentrations of tumor necrosis factor, interleukin-1, and interleukin-6 in conditioned medium could not account for all the growth stimulation, and activity remained after neutralization of tumor necrosis factor, interleukin-1, and interleukin-6 with antibodies. Conclusions: Interleukin-1, interleukin-6, tumor necrosis factor, and additional monocyte factor(s) could provide paracrine growth stimulation when monocytes are attracted to ovarian cancers that produce macrophage colony-stimulating factor.

Original languageEnglish (US)
Pages (from-to)997-1007
Number of pages11
JournalAmerican Journal of Obstetrics and Gynecology
Volume166
Issue number3
DOIs
StatePublished - Mar 1992

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Ovulation Induction
Interleukin-1
Monocytes
Interleukin-6
Ovarian Neoplasms
Tumor Necrosis Factor-alpha
Cell Proliferation
Growth
Neoplasms
Macrophage Colony-Stimulating Factor
Conditioned Culture Medium
Cell Line
Tritium
Tetradecanoylphorbol Acetate
Granulocyte-Macrophage Colony-Stimulating Factor
Granulocytes
Thymidine
Lipopolysaccharides
Cell Count
Macrophages

Keywords

  • Monocyte
  • growth factor
  • ovarian cancer

ASJC Scopus subject areas

  • Obstetrics and Gynecology

Cite this

Stimulation of ovarian tumor cell proliferation with monocyte products including interleukin-1, interleukin-6, and tumor necrosis factor-α. / Wu, Shu; Rodabaugh, Kerry; Martinez-Maza, Otoniel; Watson, Joanna M.; Silberstein, David S.; Boyer, Cinda M.; Peters, William P.; Weinberg, J. Brice; Berek, Jonathan S.; Bast, Robert C.

In: American Journal of Obstetrics and Gynecology, Vol. 166, No. 3, 03.1992, p. 997-1007.

Research output: Contribution to journalArticle

Wu, Shu ; Rodabaugh, Kerry ; Martinez-Maza, Otoniel ; Watson, Joanna M. ; Silberstein, David S. ; Boyer, Cinda M. ; Peters, William P. ; Weinberg, J. Brice ; Berek, Jonathan S. ; Bast, Robert C. / Stimulation of ovarian tumor cell proliferation with monocyte products including interleukin-1, interleukin-6, and tumor necrosis factor-α. In: American Journal of Obstetrics and Gynecology. 1992 ; Vol. 166, No. 3. pp. 997-1007.
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AU - Silberstein, David S.

AU - Boyer, Cinda M.

AU - Peters, William P.

AU - Weinberg, J. Brice

AU - Berek, Jonathan S.

AU - Bast, Robert C.

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