Stimulation of Na+-K+-ATPase by thyrotropin in cultured thyroid follicular cells

T. A. Pressley, S. C. Higham, L. A. Joson, D. W. Mercer

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Thyroid-stimulating hormone (TSH; thyrotropin) produces a pleiotropic response in the thyroid gland, accelerating nearly every aspect of metabolic turnover within the follicular epithelia. We examined the effects of TSH on expression of Na+-K+-ATPase in FRTL-5 cells, a cell line derived from rat thyroid. TSH (10 mU/ml) produced a nearly twofold increase in abundance of the mRNA encoding the catalytic α1-subunit within 6 h of treatment. With the four mRNAs encoding the β1-subunit, TSH produced a striking increase in abundance, but this regulation was discoordinate, and some species increased more than others. Similar increases in mRNA abundance were elicited by activators of the adenosine 3',5'-cyclic monophosphate second messenger system. In contrast to the α1- and β1-mRNAs, the abundance of the mRNA encoding the β2-subunit was unchanged with TSH after 6 h, indicating that the effects of thyrotropin were not universal or indiscriminate. Thyrotropin also caused a 76% increase in Na+-K+-ATPase activity and a 46% increase in pump-mediated transport after 48 h. These studies suggest that the changes in metabolic turnover initiated by TSH during hormone synthesis include upregulation of the Na+-K+ pump.

Original languageEnglish (US)
Pages (from-to)C1252-C1258
JournalAmerican Journal of Physiology - Cell Physiology
Volume268
Issue number5 37-5
StatePublished - Jan 1 1995

Fingerprint

thyrotropin
sodium-potassium-exchanging ATPase
Thyrotropin
Adenosine Triphosphatases
Cultured Cells
Messenger RNA
cells
Thyroid Gland
Pumps
thyroid gland
second messengers
cyclic AMP
pumps
Second Messenger Systems
epithelium
Cyclic AMP
cell lines
hormones
Rats
Catalytic Domain

Keywords

  • adenosine 3',5'- cyclic monophosphate
  • ion transport
  • sodium-potassium pump
  • thyroid regulation

ASJC Scopus subject areas

  • Physiology
  • Cell Biology

Cite this

Stimulation of Na+-K+-ATPase by thyrotropin in cultured thyroid follicular cells. / Pressley, T. A.; Higham, S. C.; Joson, L. A.; Mercer, D. W.

In: American Journal of Physiology - Cell Physiology, Vol. 268, No. 5 37-5, 01.01.1995, p. C1252-C1258.

Research output: Contribution to journalArticle

Pressley, T. A. ; Higham, S. C. ; Joson, L. A. ; Mercer, D. W. / Stimulation of Na+-K+-ATPase by thyrotropin in cultured thyroid follicular cells. In: American Journal of Physiology - Cell Physiology. 1995 ; Vol. 268, No. 5 37-5. pp. C1252-C1258.
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