Staurosporine blocks down-regulation of monocyte-associated tissue factor

J. P. Brozna, M. Forman, S. D. Carson

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Inflammatory agents including bacterial endotoxin (LPS) and low concentrations of phorbol myristate acetate (PMA) stimulate human peripheral blood monocytes to transiently express tissue factor procoagulant activity. Concentrations of PMA that cause the cytosol-to-membrane translocation of protein kinase C (PKC)) (10-9-10-7 M) induce a rapid decrease in monocyte tissue factor activity. Staurosporine, an inhibitor of protein kinase C, enhances the stimulatory effect of low concentrations of PMA on monocyte expression of tissue factor activity and blocks suppression of tissue factor activity at high PMA concentrations. Furthermore, staurosporine prolongs LPS-induced tissue factor expression in monocytes. These results suggest that protein kinases modulate tissue factor activity in human monocytes by regulating both induction and down-regulation.

Original languageEnglish (US)
Pages (from-to)929-938
Number of pages10
JournalBlood Coagulation and Fibrinolysis
Volume5
Issue number6
Publication statusPublished - Dec 1 1994

    Fingerprint

Keywords

  • Monocytes
  • Protein kinase C
  • Tissue factor

ASJC Scopus subject areas

  • Hematology

Cite this