State of the art and future needs in cytogenetic/molecular genetics/arrays in childhood lymphoma: Summary report of workshop at the First International Symposium on Childhood and Adolescent Non-Hodgkin Lymphoma, April 9, 2003, New York City, NY

Nyla A. Heerema, Alain Bernheim, Megan S. Lim, A. Thomas Look, Laura Pasqualucci, Elizabeth Raetz, Warren G. Sanger, Mitchell S. Cairo

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Background. A significant number of studies describe the cytogenetics and molecular genetics of adult non-Hodgkin lymphoma (NHL); however, similar knowledge is lacking regarding pediatric NHL. Methods. A workshop to discuss the "State of the Art and Future Needs in Cytogenetic/Molecular Genetics/Arrays" in pediatric NHL was held in conjunction with the First International Symposium on Childhood and Adolescent Non-Hodgkin Lymphoma on April 9, 2003 in New York City. Results. Cytogenetic characteristics of pediatric NHL include 14q11.2 rearrangements in T-cell lymphoblastic leukemia/lymphomas (LBL), ALK rearrangements in anaplastic large cell lymphomas (ALCL), and CMYC translocations in both Burkitt and Burkitt-like lymphomas (BL/BLL). Pediatric diffuse large B-cell lymphoma (DLBCL) is cytogenetically different from DLBCL in adults, suggesting a different disease in children. Microarray studies demonstrate three types of T-cell leukemia, the leukemic counterpart of LBL, that block T-cell differentiation at different stages of T-cell development, corresponding to LYL, TAL1, and HOX-expressing leukemias. ALCL cell lines have a unique expression profile compared to normal T-cells. Germinal centers of BL have CMYC expression signatures, indicating that CMYC expression is ectopic and does not reflect the physiology of the normal cell counterpart. Conclusions. Additional cytogenetic, molecular and microarray investigations of NHL in children are vital to better understand these diseases, their etiology, and differences from adult NHL. A greater understanding of pediatric NHL will lead to disease-specific and patient-individualized therapies of these diseases.

Original languageEnglish (US)
Pages (from-to)616-622
Number of pages7
JournalPediatric Blood and Cancer
Volume45
Issue number5
DOIs
StatePublished - Oct 15 2005

Fingerprint

Cytogenetics
Non-Hodgkin's Lymphoma
Molecular Biology
Lymphoma
Education
Precursor Cell Lymphoblastic Leukemia-Lymphoma
Pediatrics
Anaplastic Large-Cell Lymphoma
T-Cell Leukemia
Lymphoma, Large B-Cell, Diffuse
T-Lymphocytes
Hodgkin Disease
Cell Physiological Phenomena
Germinal Center
Burkitt Lymphoma
Cell Differentiation
Leukemia
Cell Line

Keywords

  • Childhood
  • Chromosomes
  • Microarray
  • Molecular
  • Non-Hodgkin lymphoma

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Hematology
  • Oncology

Cite this

State of the art and future needs in cytogenetic/molecular genetics/arrays in childhood lymphoma : Summary report of workshop at the First International Symposium on Childhood and Adolescent Non-Hodgkin Lymphoma, April 9, 2003, New York City, NY. / Heerema, Nyla A.; Bernheim, Alain; Lim, Megan S.; Look, A. Thomas; Pasqualucci, Laura; Raetz, Elizabeth; Sanger, Warren G.; Cairo, Mitchell S.

In: Pediatric Blood and Cancer, Vol. 45, No. 5, 15.10.2005, p. 616-622.

Research output: Contribution to journalArticle

Heerema, Nyla A. ; Bernheim, Alain ; Lim, Megan S. ; Look, A. Thomas ; Pasqualucci, Laura ; Raetz, Elizabeth ; Sanger, Warren G. ; Cairo, Mitchell S. / State of the art and future needs in cytogenetic/molecular genetics/arrays in childhood lymphoma : Summary report of workshop at the First International Symposium on Childhood and Adolescent Non-Hodgkin Lymphoma, April 9, 2003, New York City, NY. In: Pediatric Blood and Cancer. 2005 ; Vol. 45, No. 5. pp. 616-622.
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T1 - State of the art and future needs in cytogenetic/molecular genetics/arrays in childhood lymphoma

T2 - Summary report of workshop at the First International Symposium on Childhood and Adolescent Non-Hodgkin Lymphoma, April 9, 2003, New York City, NY

AU - Heerema, Nyla A.

AU - Bernheim, Alain

AU - Lim, Megan S.

AU - Look, A. Thomas

AU - Pasqualucci, Laura

AU - Raetz, Elizabeth

AU - Sanger, Warren G.

AU - Cairo, Mitchell S.

PY - 2005/10/15

Y1 - 2005/10/15

N2 - Background. A significant number of studies describe the cytogenetics and molecular genetics of adult non-Hodgkin lymphoma (NHL); however, similar knowledge is lacking regarding pediatric NHL. Methods. A workshop to discuss the "State of the Art and Future Needs in Cytogenetic/Molecular Genetics/Arrays" in pediatric NHL was held in conjunction with the First International Symposium on Childhood and Adolescent Non-Hodgkin Lymphoma on April 9, 2003 in New York City. Results. Cytogenetic characteristics of pediatric NHL include 14q11.2 rearrangements in T-cell lymphoblastic leukemia/lymphomas (LBL), ALK rearrangements in anaplastic large cell lymphomas (ALCL), and CMYC translocations in both Burkitt and Burkitt-like lymphomas (BL/BLL). Pediatric diffuse large B-cell lymphoma (DLBCL) is cytogenetically different from DLBCL in adults, suggesting a different disease in children. Microarray studies demonstrate three types of T-cell leukemia, the leukemic counterpart of LBL, that block T-cell differentiation at different stages of T-cell development, corresponding to LYL, TAL1, and HOX-expressing leukemias. ALCL cell lines have a unique expression profile compared to normal T-cells. Germinal centers of BL have CMYC expression signatures, indicating that CMYC expression is ectopic and does not reflect the physiology of the normal cell counterpart. Conclusions. Additional cytogenetic, molecular and microarray investigations of NHL in children are vital to better understand these diseases, their etiology, and differences from adult NHL. A greater understanding of pediatric NHL will lead to disease-specific and patient-individualized therapies of these diseases.

AB - Background. A significant number of studies describe the cytogenetics and molecular genetics of adult non-Hodgkin lymphoma (NHL); however, similar knowledge is lacking regarding pediatric NHL. Methods. A workshop to discuss the "State of the Art and Future Needs in Cytogenetic/Molecular Genetics/Arrays" in pediatric NHL was held in conjunction with the First International Symposium on Childhood and Adolescent Non-Hodgkin Lymphoma on April 9, 2003 in New York City. Results. Cytogenetic characteristics of pediatric NHL include 14q11.2 rearrangements in T-cell lymphoblastic leukemia/lymphomas (LBL), ALK rearrangements in anaplastic large cell lymphomas (ALCL), and CMYC translocations in both Burkitt and Burkitt-like lymphomas (BL/BLL). Pediatric diffuse large B-cell lymphoma (DLBCL) is cytogenetically different from DLBCL in adults, suggesting a different disease in children. Microarray studies demonstrate three types of T-cell leukemia, the leukemic counterpart of LBL, that block T-cell differentiation at different stages of T-cell development, corresponding to LYL, TAL1, and HOX-expressing leukemias. ALCL cell lines have a unique expression profile compared to normal T-cells. Germinal centers of BL have CMYC expression signatures, indicating that CMYC expression is ectopic and does not reflect the physiology of the normal cell counterpart. Conclusions. Additional cytogenetic, molecular and microarray investigations of NHL in children are vital to better understand these diseases, their etiology, and differences from adult NHL. A greater understanding of pediatric NHL will lead to disease-specific and patient-individualized therapies of these diseases.

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KW - Molecular

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