Staphylococcus epidermidis polysaccharide intercellular adhesin production significantly increases during tricarboxylic acid cycle stress

Cuong Vuong, Joshua B. Kidder, Erik R. Jacobson, Michael Otto, Richard A. Proctor, Greg A Somerville

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

Staphylococcal polysaccharide intercellular adhesim (PIA) is important for the development of a mature biofilm. PIA production is increased during growth in a nutrient-replete or iron-limited medium and under conditions of low oxygen availability. Additionally, stress-inducing stimuli such as heat, ethanol, and high concentrations of salt increase the production of PIA. These same environmental conditions are known to repress tricarboxylic acid (TCA) cycle activity, leading us to hypothesize that altering TCA cycle activity would affect PIA production. Culturing Staphylococcus epidermidis with a low concentration of the TCA cycle inhibitor fluorocitrate dramatically increased PIA production without impairing glucose catabolism, the growth rate, or the growth yields. These data lead us to speculate that one mechanism by which staphylococci perceive external environmental change is through alterations in TCA cycle activity leading to changes in the intracellular levels of biosynthetic intermediates, ATP, or the redox status of the cell. These changes in the metabolic status of the bacteria result in the attenuation or augmentation of PIA production.

Original languageEnglish (US)
Pages (from-to)2967-2973
Number of pages7
JournalJournal of bacteriology
Volume187
Issue number9
DOIs
StatePublished - May 1 2005

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Citric Acid Cycle
Staphylococcus epidermidis
Polysaccharides
Growth
Biofilms
Staphylococcus
Oxidation-Reduction
polysaccharide intercellular adhesin
Ethanol
Iron
Salts
Hot Temperature
Adenosine Triphosphate
Oxygen
Bacteria
Glucose
Food

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

Staphylococcus epidermidis polysaccharide intercellular adhesin production significantly increases during tricarboxylic acid cycle stress. / Vuong, Cuong; Kidder, Joshua B.; Jacobson, Erik R.; Otto, Michael; Proctor, Richard A.; Somerville, Greg A.

In: Journal of bacteriology, Vol. 187, No. 9, 01.05.2005, p. 2967-2973.

Research output: Contribution to journalArticle

Vuong, Cuong ; Kidder, Joshua B. ; Jacobson, Erik R. ; Otto, Michael ; Proctor, Richard A. ; Somerville, Greg A. / Staphylococcus epidermidis polysaccharide intercellular adhesin production significantly increases during tricarboxylic acid cycle stress. In: Journal of bacteriology. 2005 ; Vol. 187, No. 9. pp. 2967-2973.
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