Staphylococcus aureus domain V functions in Escherichia coli ribosomes provided a conserved interaction with domain IV is restored

Jill Thompson, William E. Tapprich, Corey Munger, Albert E. Dahlberg

Research output: Contribution to journalArticle

6 Scopus citations


Domain V of Escherichia coli 23 S rRNA (residues 2023-2630) was replaced by that from Staphylococcus aureus, thereby introducing 132 changes in the rRNA sequence. The resulting ribosomal mutant was unable to support cell growth. The mutant was rescued, however, by restoring an interaction between domains IV and V (residues 1782 and 2586). Although the importance of this interaction, U/U in E. coli, C/C in S. aureus, is therefore demonstrated, it cannot be the only tertiary interaction important for ribosomal function as the rescued hybrid grew more slowly than the wild type. Additionally, although the single-site mutations U1782C and U2586C in E. coli are viable, the double mutant is lethal.

Original languageEnglish (US)
Pages (from-to)1076-1083
Number of pages8
Issue number8
StatePublished - Aug 20 2001



  • Hybrid ribosomes
  • Large ribosomal subunit
  • Pathogenic bacteria
  • Peptidyl transferase center
  • Ribosomal RNA

ASJC Scopus subject areas

  • Molecular Biology

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