Staphylococcus aureus CodY negatively regulates virulence gene expression

Charlotte D. Majerczyk, Marat R. Sadykov, Thanh T. Luong, Chia Lee, Greg A Somerville, Abraham L. Sonenshein

Research output: Contribution to journalArticle

109 Citations (Scopus)

Abstract

CodY is a global regulatory protein that was first discovered in Bacillus subtilis, where it couples gene expression to changes in the pools of critical metabolites through its activation by GTP and branched-chain amino acids. Homologs of CodY can be found encoded in the genomes of nearly all low-G+C gram-positive bacteria, including Staphylococcus aureus. The introduction of a codY-null mutation into two S. aureus clinical isolates, SA564 and UAMS-1, through allelic replacement, resulted in the overexpression of several virulence genes. The mutant strains had higher levels of hemolytic activity toward rabbit erythrocytes in their culture fluid, produced more polysaccharide intercellular adhesin (PIA), and formed more robust biofilms than did their isogenic parent strains. These phenotypes were associated with derepressed levels of RNA for the hemolytic alpha-toxin (hla), the accessory gene regulator (agr) (RNAII and RNAIII/hld), and the operon responsible for the production of PIA (icaADBC). These data suggest that CodY represses, either directly or indirectly, the synthesis of a number of virulence factors of S. aureus.

Original languageEnglish (US)
Pages (from-to)2257-2265
Number of pages9
JournalJournal of bacteriology
Volume190
Issue number7
DOIs
StatePublished - Apr 1 2008

Fingerprint

Virulence
Staphylococcus aureus
Gene Expression
Branched Chain Amino Acids
Gram-Positive Bacteria
Virulence Factors
Regulator Genes
Operon
Biofilms
Guanosine Triphosphate
Bacillus subtilis
Erythrocytes
Genome
RNA
Rabbits
Phenotype
Mutation
Genes
Proteins
polysaccharide intercellular adhesin

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

Majerczyk, C. D., Sadykov, M. R., Luong, T. T., Lee, C., Somerville, G. A., & Sonenshein, A. L. (2008). Staphylococcus aureus CodY negatively regulates virulence gene expression. Journal of bacteriology, 190(7), 2257-2265. https://doi.org/10.1128/JB.01545-07

Staphylococcus aureus CodY negatively regulates virulence gene expression. / Majerczyk, Charlotte D.; Sadykov, Marat R.; Luong, Thanh T.; Lee, Chia; Somerville, Greg A; Sonenshein, Abraham L.

In: Journal of bacteriology, Vol. 190, No. 7, 01.04.2008, p. 2257-2265.

Research output: Contribution to journalArticle

Majerczyk, CD, Sadykov, MR, Luong, TT, Lee, C, Somerville, GA & Sonenshein, AL 2008, 'Staphylococcus aureus CodY negatively regulates virulence gene expression', Journal of bacteriology, vol. 190, no. 7, pp. 2257-2265. https://doi.org/10.1128/JB.01545-07
Majerczyk, Charlotte D. ; Sadykov, Marat R. ; Luong, Thanh T. ; Lee, Chia ; Somerville, Greg A ; Sonenshein, Abraham L. / Staphylococcus aureus CodY negatively regulates virulence gene expression. In: Journal of bacteriology. 2008 ; Vol. 190, No. 7. pp. 2257-2265.
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