Staphylococcus aureus ClpC is required for stress resistance, aconitase activity, growth recovery, and death

Indranil Chatterjee, Petra Becker, Matthias Grundmeier, Markus Bischoff, Greg A Somerville, Georg Peters, Bhanu Sinha, Niamh Harraghy, Richard A. Proctor, Mathias Herrmann

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

The ability of Staphylococcus aureus to adapt to various conditions of stress is the result of a complex regulatory response. Previously, it has been demonstrated that Clp homologues are important for a variety of stress conditions, and our laboratory has shown that a clpC homologue was highly expressed in the S. aureus strain DSM20231 during biofilm formation relative to expression in planktonic cells. Persistence and long-term survival are a hallmark of biofilm-associated staphylococcal infections, as cure frequently fails even in the presence of bactericidal antimicrobials. To determine the role of clpC in this context, we performed metabolic, gene expression, and long-term growth and survival analyses of DSM20231 as well as an isogenic clpC allelic-replacement mutant, a sigB mutant, and a clpC sigB double mutant. As expected, the clpC mutant showed increased sensitivity to oxidative and heat stresses. Unanticipated, however, was the reduced expression of the tricarboxylic acid (TCA) cycle gene citB (encoding aconitase), resulting in the loss of aconitase activity and preventing the catabolization of acetate during the stationary phase. clpC inactivation abolished post-stationary-phase recovery but also resulted in significantly enhanced stationary-phase survival compared to that of the wild-type strain. These data demonstrate the critical role of the ClpC ATPase in regulating the TCA cycle and implicate ClpC as being important for recovery from the stationary phase and also for entering the death phase. Understanding the stationary- and post-stationary-phase recovery in S. aureus may have important clinical implications, as little is known about the mechanisms of long-term persistence of chronic S. aureus infections associated with formation of biofilms.

Original languageEnglish (US)
Pages (from-to)4488-4496
Number of pages9
JournalJournal of bacteriology
Volume187
Issue number13
DOIs
StatePublished - Jul 1 2005

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Aconitate Hydratase
Staphylococcus aureus
Biofilms
Citric Acid Cycle
Growth
Staphylococcal Infections
Survival Analysis
Adenosine Triphosphatases
Acetates
Oxidative Stress
Hot Temperature
Gene Expression
Infection
Genes

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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Staphylococcus aureus ClpC is required for stress resistance, aconitase activity, growth recovery, and death. / Chatterjee, Indranil; Becker, Petra; Grundmeier, Matthias; Bischoff, Markus; Somerville, Greg A; Peters, Georg; Sinha, Bhanu; Harraghy, Niamh; Proctor, Richard A.; Herrmann, Mathias.

In: Journal of bacteriology, Vol. 187, No. 13, 01.07.2005, p. 4488-4496.

Research output: Contribution to journalArticle

Chatterjee, I, Becker, P, Grundmeier, M, Bischoff, M, Somerville, GA, Peters, G, Sinha, B, Harraghy, N, Proctor, RA & Herrmann, M 2005, 'Staphylococcus aureus ClpC is required for stress resistance, aconitase activity, growth recovery, and death', Journal of bacteriology, vol. 187, no. 13, pp. 4488-4496. https://doi.org/10.1128/JB.187.13.4488-4496.2005
Chatterjee, Indranil ; Becker, Petra ; Grundmeier, Matthias ; Bischoff, Markus ; Somerville, Greg A ; Peters, Georg ; Sinha, Bhanu ; Harraghy, Niamh ; Proctor, Richard A. ; Herrmann, Mathias. / Staphylococcus aureus ClpC is required for stress resistance, aconitase activity, growth recovery, and death. In: Journal of bacteriology. 2005 ; Vol. 187, No. 13. pp. 4488-4496.
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AU - Somerville, Greg A

AU - Peters, Georg

AU - Sinha, Bhanu

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