Staphylococcus aureus ClpC ATPase is a late growth phase effector of metabolism and persistence

Indranil Chatterjee, Sigrid Schmitt, Christoph F. Batzilla, Susanne Engelmann, Andreas Keller, Michael W. Ring, Ralf Kautenburger, Wilma Ziebuhr, Michael Hecker, Klaus T. Preissner, Markus Bischoff, Richard A. Proctor, Horst P. Beck, Hans Peter Lenhof, Greg A Somerville, Mathias Herrmann

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Staphylococcus aureus Clp ATPases (molecular chaperones) alter normal physiological functions including an aconitase-mediated effect onpost-stationary growth, acetate catabolism, and entry into death phase (Chatterjee et al., J. Bacteriol. 2005, 187, 4488-4496). In the present study, the global function of ClpC in physiology, metabolism, and late-stationary phase survival was examined using DNA microarrays and 2-D PAGE followed by MALDI-TOF MS. The results suggest that ClpC is involved in regulating the expression of genes and/or proteins of gluconeogenesis, the pentose-phosphate pathway, pyruvate metabolism, the electron transport chain, nucleotide metabolism, oxidative stress, metal ion homeostasis, stringent response, and programmed cell death. Thus, one major function of ClpC is balancing late growth phase carbon metabolism. Furthermore, these changes in carbon metabolismresult in alterations of the intracellular concentration of free NADH, the amount of cell-associated iron, and fatty acid metabolism. This study provides strong evidence for ClpC as a critical factor in staphylococcal energy metabolism, stress regulation, and late-stationary phase survival; therefore, these data provide important insight into the adaptation of S. aureus toward a persister state in chronic infections.

Original languageEnglish (US)
Pages (from-to)1152-1176
Number of pages25
JournalProteomics
Volume9
Issue number5
DOIs
StatePublished - Mar 1 2009

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Metabolism
Adenosine Triphosphatases
Staphylococcus aureus
Growth
Carbon
Aconitate Hydratase
Pentoses
Molecular Chaperones
Oxidative stress
Physiology
Cell death
Pentose Phosphate Pathway
Microarrays
Pyruvic Acid
Gluconeogenesis
NAD
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Metal ions
Electron Transport
Oligonucleotide Array Sequence Analysis

Keywords

  • ClpC
  • Metabolism
  • Persistence
  • Staphylococcus aureus
  • Tricarboxylic acid cycle

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Chatterjee, I., Schmitt, S., Batzilla, C. F., Engelmann, S., Keller, A., Ring, M. W., ... Herrmann, M. (2009). Staphylococcus aureus ClpC ATPase is a late growth phase effector of metabolism and persistence. Proteomics, 9(5), 1152-1176. https://doi.org/10.1002/pmic.200800586

Staphylococcus aureus ClpC ATPase is a late growth phase effector of metabolism and persistence. / Chatterjee, Indranil; Schmitt, Sigrid; Batzilla, Christoph F.; Engelmann, Susanne; Keller, Andreas; Ring, Michael W.; Kautenburger, Ralf; Ziebuhr, Wilma; Hecker, Michael; Preissner, Klaus T.; Bischoff, Markus; Proctor, Richard A.; Beck, Horst P.; Lenhof, Hans Peter; Somerville, Greg A; Herrmann, Mathias.

In: Proteomics, Vol. 9, No. 5, 01.03.2009, p. 1152-1176.

Research output: Contribution to journalArticle

Chatterjee, I, Schmitt, S, Batzilla, CF, Engelmann, S, Keller, A, Ring, MW, Kautenburger, R, Ziebuhr, W, Hecker, M, Preissner, KT, Bischoff, M, Proctor, RA, Beck, HP, Lenhof, HP, Somerville, GA & Herrmann, M 2009, 'Staphylococcus aureus ClpC ATPase is a late growth phase effector of metabolism and persistence', Proteomics, vol. 9, no. 5, pp. 1152-1176. https://doi.org/10.1002/pmic.200800586
Chatterjee I, Schmitt S, Batzilla CF, Engelmann S, Keller A, Ring MW et al. Staphylococcus aureus ClpC ATPase is a late growth phase effector of metabolism and persistence. Proteomics. 2009 Mar 1;9(5):1152-1176. https://doi.org/10.1002/pmic.200800586
Chatterjee, Indranil ; Schmitt, Sigrid ; Batzilla, Christoph F. ; Engelmann, Susanne ; Keller, Andreas ; Ring, Michael W. ; Kautenburger, Ralf ; Ziebuhr, Wilma ; Hecker, Michael ; Preissner, Klaus T. ; Bischoff, Markus ; Proctor, Richard A. ; Beck, Horst P. ; Lenhof, Hans Peter ; Somerville, Greg A ; Herrmann, Mathias. / Staphylococcus aureus ClpC ATPase is a late growth phase effector of metabolism and persistence. In: Proteomics. 2009 ; Vol. 9, No. 5. pp. 1152-1176.
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