3 Citations (Scopus)

Abstract

Background: Loss of alveolar ridge width and height after tooth extraction is well documented, but models to evaluate ridge preservation are neither standardized nor cost-effective. This rat model characterizes the pattern of bone turnover and inflammation after extraction and bone grafting with or without local simvastatin (SIM). Methods: Fifty retired-breeder rats underwent extraction of the maxillary right first molar and standard surgical defect creation under inhalation/local anesthesia. The left side of each animal served as unmanipulated control. Untreated groups (n = 8 to 9 per group) were compared (analysis of variance, t test) at days 0, 7, 14, and 28 for alveolar ridge height and width and for markers of inflammation and bone turnover by microcomputed tomography, histology, and enzyme-linked immunosorbent assay. Seventeen additional specimens had defects grafted with either bone mineralized matrix (BMM) or a BMM+SIM conjugate. Results: Extraction-induced bone loss (BL) was noted on buccal, palatal, and interproximal height (P <0.05) and ridge width (P <0.01). Week 1 inflammation positively correlated with ridge height; thereafter, a more intense inflammatory reaction corresponded to reduction in alveolar bone height and density (r = 0.74; P <0.05; Spearman). BMM+SIM preserved the most interproximal bone height (P <0.01), increased ridge width and bone density (P <0.01), enhanced 7-day prostaglandin E2 (P <0.01), and reduced 28-day inflammation density (P <0.05). Conclusions: The standard defect used in the current study paralleled human postextraction alveolar BL. Defect grafting, especially BMM+SIM, reduced inflammation and preserved bone.

Original languageEnglish (US)
Pages (from-to)799-807
Number of pages9
JournalJournal of Periodontology
Volume88
Issue number8
DOIs
StatePublished - Aug 1 2017

Fingerprint

Bone Matrix
Simvastatin
Inflammation
Alveolar Process
Osteitis
Bone Remodeling
Bone Density
Inhalation Anesthesia
Alveolar Bone Loss
Bone and Bones
X-Ray Microtomography
Tooth Extraction
Bone Transplantation
Cheek
Local Anesthesia
Dinoprostone
Histology
Analysis of Variance
Enzyme-Linked Immunosorbent Assay
Costs and Cost Analysis

Keywords

  • Bone remodeling
  • Inflammation
  • Rats
  • Tooth extraction

ASJC Scopus subject areas

  • Periodontics

Cite this

Standardized rat model testing effects of inflammation and grafting on extraction healing. / Willett, Emily S.; Liu, Jingpeng; Berke, Molly; Giannini, Peter J; Schmid, Marian; Jia, Zhenshan; Wang, Xiaobei; Wang, Xiaoyan; Samson, Kaeli; Yu, Fang; Wang, Dong; Nawshad, Ali; Reinhardt, Richard A.

In: Journal of Periodontology, Vol. 88, No. 8, 01.08.2017, p. 799-807.

Research output: Contribution to journalArticle

Willett, Emily S. ; Liu, Jingpeng ; Berke, Molly ; Giannini, Peter J ; Schmid, Marian ; Jia, Zhenshan ; Wang, Xiaobei ; Wang, Xiaoyan ; Samson, Kaeli ; Yu, Fang ; Wang, Dong ; Nawshad, Ali ; Reinhardt, Richard A. / Standardized rat model testing effects of inflammation and grafting on extraction healing. In: Journal of Periodontology. 2017 ; Vol. 88, No. 8. pp. 799-807.
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abstract = "Background: Loss of alveolar ridge width and height after tooth extraction is well documented, but models to evaluate ridge preservation are neither standardized nor cost-effective. This rat model characterizes the pattern of bone turnover and inflammation after extraction and bone grafting with or without local simvastatin (SIM). Methods: Fifty retired-breeder rats underwent extraction of the maxillary right first molar and standard surgical defect creation under inhalation/local anesthesia. The left side of each animal served as unmanipulated control. Untreated groups (n = 8 to 9 per group) were compared (analysis of variance, t test) at days 0, 7, 14, and 28 for alveolar ridge height and width and for markers of inflammation and bone turnover by microcomputed tomography, histology, and enzyme-linked immunosorbent assay. Seventeen additional specimens had defects grafted with either bone mineralized matrix (BMM) or a BMM+SIM conjugate. Results: Extraction-induced bone loss (BL) was noted on buccal, palatal, and interproximal height (P <0.05) and ridge width (P <0.01). Week 1 inflammation positively correlated with ridge height; thereafter, a more intense inflammatory reaction corresponded to reduction in alveolar bone height and density (r = 0.74; P <0.05; Spearman). BMM+SIM preserved the most interproximal bone height (P <0.01), increased ridge width and bone density (P <0.01), enhanced 7-day prostaglandin E2 (P <0.01), and reduced 28-day inflammation density (P <0.05). Conclusions: The standard defect used in the current study paralleled human postextraction alveolar BL. Defect grafting, especially BMM+SIM, reduced inflammation and preserved bone.",
keywords = "Bone remodeling, Inflammation, Rats, Tooth extraction",
author = "Willett, {Emily S.} and Jingpeng Liu and Molly Berke and Giannini, {Peter J} and Marian Schmid and Zhenshan Jia and Xiaobei Wang and Xiaoyan Wang and Kaeli Samson and Fang Yu and Dong Wang and Ali Nawshad and Reinhardt, {Richard A}",
year = "2017",
month = "8",
day = "1",
doi = "10.1902/jop.2017.160771",
language = "English (US)",
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TY - JOUR

T1 - Standardized rat model testing effects of inflammation and grafting on extraction healing

AU - Willett, Emily S.

AU - Liu, Jingpeng

AU - Berke, Molly

AU - Giannini, Peter J

AU - Schmid, Marian

AU - Jia, Zhenshan

AU - Wang, Xiaobei

AU - Wang, Xiaoyan

AU - Samson, Kaeli

AU - Yu, Fang

AU - Wang, Dong

AU - Nawshad, Ali

AU - Reinhardt, Richard A

PY - 2017/8/1

Y1 - 2017/8/1

N2 - Background: Loss of alveolar ridge width and height after tooth extraction is well documented, but models to evaluate ridge preservation are neither standardized nor cost-effective. This rat model characterizes the pattern of bone turnover and inflammation after extraction and bone grafting with or without local simvastatin (SIM). Methods: Fifty retired-breeder rats underwent extraction of the maxillary right first molar and standard surgical defect creation under inhalation/local anesthesia. The left side of each animal served as unmanipulated control. Untreated groups (n = 8 to 9 per group) were compared (analysis of variance, t test) at days 0, 7, 14, and 28 for alveolar ridge height and width and for markers of inflammation and bone turnover by microcomputed tomography, histology, and enzyme-linked immunosorbent assay. Seventeen additional specimens had defects grafted with either bone mineralized matrix (BMM) or a BMM+SIM conjugate. Results: Extraction-induced bone loss (BL) was noted on buccal, palatal, and interproximal height (P <0.05) and ridge width (P <0.01). Week 1 inflammation positively correlated with ridge height; thereafter, a more intense inflammatory reaction corresponded to reduction in alveolar bone height and density (r = 0.74; P <0.05; Spearman). BMM+SIM preserved the most interproximal bone height (P <0.01), increased ridge width and bone density (P <0.01), enhanced 7-day prostaglandin E2 (P <0.01), and reduced 28-day inflammation density (P <0.05). Conclusions: The standard defect used in the current study paralleled human postextraction alveolar BL. Defect grafting, especially BMM+SIM, reduced inflammation and preserved bone.

AB - Background: Loss of alveolar ridge width and height after tooth extraction is well documented, but models to evaluate ridge preservation are neither standardized nor cost-effective. This rat model characterizes the pattern of bone turnover and inflammation after extraction and bone grafting with or without local simvastatin (SIM). Methods: Fifty retired-breeder rats underwent extraction of the maxillary right first molar and standard surgical defect creation under inhalation/local anesthesia. The left side of each animal served as unmanipulated control. Untreated groups (n = 8 to 9 per group) were compared (analysis of variance, t test) at days 0, 7, 14, and 28 for alveolar ridge height and width and for markers of inflammation and bone turnover by microcomputed tomography, histology, and enzyme-linked immunosorbent assay. Seventeen additional specimens had defects grafted with either bone mineralized matrix (BMM) or a BMM+SIM conjugate. Results: Extraction-induced bone loss (BL) was noted on buccal, palatal, and interproximal height (P <0.05) and ridge width (P <0.01). Week 1 inflammation positively correlated with ridge height; thereafter, a more intense inflammatory reaction corresponded to reduction in alveolar bone height and density (r = 0.74; P <0.05; Spearman). BMM+SIM preserved the most interproximal bone height (P <0.01), increased ridge width and bone density (P <0.01), enhanced 7-day prostaglandin E2 (P <0.01), and reduced 28-day inflammation density (P <0.05). Conclusions: The standard defect used in the current study paralleled human postextraction alveolar BL. Defect grafting, especially BMM+SIM, reduced inflammation and preserved bone.

KW - Bone remodeling

KW - Inflammation

KW - Rats

KW - Tooth extraction

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U2 - 10.1902/jop.2017.160771

DO - 10.1902/jop.2017.160771

M3 - Article

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