Stage-dependent gene expression profiles during natural killer cell development

Hyung Sik Kang, Eun Mi Kim, Sanggyu Lee, Suk Ran Yoon, Toshihiko Kawamura, Young Cheol Lee, Sangsoo Kim, Pyung Keun Myung, San Ming Wang, Inpyo Choi

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Natural killer (NK) cells develop from hematopoietic stem cells (HSCs) in the bone marrow. To understand the molecular regulation of NK cell development, serial analysis of gene expression (SAGE) was applied to HSCs, NK precursor (pNK) cells, and mature NK cells (mNK) cultured without or with OP9 stromal cells. From 170,464 total individual tags from four SAGE libraries, 35,385 unique genes were identified. A set of genes was expressed in a stage-specific manner: 15 genes in HSCs, 30 genes in pNK cells, and 27 genes in mNK cells. Among them, lipoprotein lipase induced NK cell maturation and cytotoxic activity. Identification of genome-wide profiles of gene expression in different stages of NK cell development affords us a fundamental basis for defining the molecular network during NK cell development.

Original languageEnglish (US)
Pages (from-to)551-565
Number of pages15
JournalGenomics
Volume86
Issue number5
DOIs
StatePublished - Nov 1 2005

Fingerprint

Transcriptome
Natural Killer Cells
Hematopoietic Stem Cells
Genes
Gene Expression
Lipoprotein Lipase
Stromal Cells
Gene Library
Bone Marrow
Genome

Keywords

  • Development
  • LPL
  • NK cells
  • SAGE

ASJC Scopus subject areas

  • Genetics

Cite this

Kang, H. S., Kim, E. M., Lee, S., Yoon, S. R., Kawamura, T., Lee, Y. C., ... Choi, I. (2005). Stage-dependent gene expression profiles during natural killer cell development. Genomics, 86(5), 551-565. https://doi.org/10.1016/j.ygeno.2005.06.010

Stage-dependent gene expression profiles during natural killer cell development. / Kang, Hyung Sik; Kim, Eun Mi; Lee, Sanggyu; Yoon, Suk Ran; Kawamura, Toshihiko; Lee, Young Cheol; Kim, Sangsoo; Myung, Pyung Keun; Wang, San Ming; Choi, Inpyo.

In: Genomics, Vol. 86, No. 5, 01.11.2005, p. 551-565.

Research output: Contribution to journalArticle

Kang, HS, Kim, EM, Lee, S, Yoon, SR, Kawamura, T, Lee, YC, Kim, S, Myung, PK, Wang, SM & Choi, I 2005, 'Stage-dependent gene expression profiles during natural killer cell development', Genomics, vol. 86, no. 5, pp. 551-565. https://doi.org/10.1016/j.ygeno.2005.06.010
Kang HS, Kim EM, Lee S, Yoon SR, Kawamura T, Lee YC et al. Stage-dependent gene expression profiles during natural killer cell development. Genomics. 2005 Nov 1;86(5):551-565. https://doi.org/10.1016/j.ygeno.2005.06.010
Kang, Hyung Sik ; Kim, Eun Mi ; Lee, Sanggyu ; Yoon, Suk Ran ; Kawamura, Toshihiko ; Lee, Young Cheol ; Kim, Sangsoo ; Myung, Pyung Keun ; Wang, San Ming ; Choi, Inpyo. / Stage-dependent gene expression profiles during natural killer cell development. In: Genomics. 2005 ; Vol. 86, No. 5. pp. 551-565.
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